Recombinant pan Keratin antibody (pan)

Catalog No. ABIN7879692

The Rabbit Monoclonal anti-pan Keratin antibody is suitable to detect pan Keratin in samples from Human. It has been validated for IHC (p).

Quick Overview for Recombinant pan Keratin antibody (pan) (ABIN7879692)

Target: pan Keratin (panKRT)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: KRTL-1577R-KRTH-1576R

Product Details anti-pan Keratin Antibody

Binding Specificity: pan

Purpose: Recombinant Pan Cytokeratin Antibody Cocktail (azide and preservative free)

Purification: Protein A affinity chromatography

Immunogen: Recombinant human KRT77 and KRT76 protein were used as the immunogen for the recombinant Pan Cytokeratin antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the recombinant Pan Cytokeratin antibody should be determined by the researcher.

. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Pan Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Pan Cytokeratin

Background: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas.