Recombinant LRP6 antibody (pSer1490)

Catalog No. ABIN7879710

The Rabbit Monoclonal anti-LRP6 antibody is suitable to detect LRP6 in samples from Human. It has been validated for WB.

Quick Overview for Recombinant LRP6 antibody (pSer1490) (ABIN7879710)

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This LRP6 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 31L42

Product Details anti-LRP6 Antibody

Binding Specificity: pSer1490

Purpose: Phospho-LRP6 (Ser1490) Antibody / Low density lipoprotein receptor related protein 6

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Phospho-LRP6 (S1490) was used as the immunogen for the Phospho-LRP6 (Ser1490) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-LRP6 (Ser1490) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-LRP6 (Ser1490) antibody at -20oC.

Target Details for LRP6

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Alternative Name: LRP6

Background: Phospho-LRP6 (Ser1490) antibody detects the phosphorylated form of Low density lipoprotein receptor related protein 6, encoded by the LRP6 gene. LRP6 is a single pass transmembrane receptor that serves as a critical co receptor in the canonical Wnt signaling pathway. This receptor works together with the Frizzled family of proteins to transmit Wnt signals into the cell, leading to stabilization of beta catenin and activation of Wnt target genes. Phosphorylation at serine 1490 is a key activation step that amplifies the signaling cascade, and Phospho-LRP6 (Ser1490) antibody provides a precise tool to study Wnt pathway activation in both normal physiology and disease contexts.

Low density lipoprotein receptor related protein 6 is phosphorylated by kinases such as GSK3 and CK1 following Wnt ligand binding. Serine 1490 is one of the critical sites required for recruitment of the cytoplasmic effector Dishevelled and for inhibition of the beta catenin destruction complex. Research using Phospho-LRP6 (Ser1490) antibody has shown that phosphorylation at this site acts as a molecular switch, turning on downstream signaling. Without phosphorylation at serine 1490, Wnt mediated gene expression is severely impaired, demonstrating the importance of this modification in developmental and disease related signaling events.

Aberrant Wnt signaling is implicated in multiple cancers, including colorectal carcinoma, breast cancer, and hepatocellular carcinoma. Overexpression or hyperactivation of LRP6 contributes to increased beta catenin stabilization, driving oncogenic transcriptional programs. Studies employing Phospho-LRP6 (Ser1490) antibody have revealed that elevated phosphorylation correlates with poor prognosis and aggressive tumor progression. Beyond cancer, abnormal regulation of LRP6 phosphorylation is also associated with bone disorders, atherosclerosis, and neurodegenerative disease, highlighting the broad relevance of this receptor.

Phospho-LRP6 (Ser1490) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting allows quantification of phosphorylated versus unphosphorylated forms, providing a readout of Wnt activation. Immunohistochemistry demonstrates tissue specific activation of Wnt signaling, while immunofluorescence visualizes membrane localized phosphorylation events in cell culture models. Researchers studying embryonic development, stem cell biology, and tumor progression use Phospho-LRP6 (Ser1490) antibody to dissect pathway activity with high specificity.

By providing validated reagents such as Phospho-LRP6 (Ser1490) antibody, NSJ Bioreagents supports investigations into Wnt pathway regulation, cancer mechanisms, and developmental biology. The ability to detect this key phosphorylation event allows scientists to better understand how external cues are transduced into cellular responses that govern growth, survival, and differentiation.

UniProt: O75581

Pathways: WNT Signaling, Tube Formation