Recombinant beta Arrestin 1 antibody (pSer412)

Catalog No. ABIN7879742

This Rabbit Monoclonal antibody specifically detects beta Arrestin 1 in WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant beta Arrestin 1 antibody (pSer412) (ABIN7879742)

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This beta Arrestin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 32A60

Product Details anti-beta Arrestin 1 Antibody

Binding Specificity: pSer412

Purpose: Phospho-Beta Arrestin 1 (Ser412) Antibody / ARRB1

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Phospho-beta Arrestin 1 (S412) was used as the immunogen for the Phospho-Beta Arrestin 1 (Ser412) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-Beta Arrestin 1 (Ser412) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-Beta Arrestin 1 (Ser412) antibody at -20oC.

Target Details for beta Arrestin 1

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Alternative Name: Beta Arrestin 1

Background: Phospho-Beta Arrestin 1 (Ser412) antibody detects Beta arrestin 1 phosphorylated at serine 412, encoded by the ARRB1 gene. Beta arrestin 1 is a multifunctional adaptor protein best known for its role in G protein coupled receptor desensitization and internalization. Phosphorylation at serine 412 represents a key regulatory modification that influences receptor trafficking, signaling scaffolds, and cellular responses to extracellular stimuli. Phospho-Beta Arrestin 1 (Ser412) antibody provides a highly specific tool for dissecting the dynamic regulation of G protein coupled receptor signaling and arrestin mediated pathways.

Beta arrestin 1 functions by binding activated, phosphorylated G protein coupled receptors, preventing further coupling to heterotrimeric G proteins while simultaneously recruiting clathrin and adaptor proteins to promote endocytosis. Research using Phospho-Beta Arrestin 1 (Ser412) antibody has demonstrated that phosphorylation at serine 412 modulates the strength and duration of this interaction, acting as a molecular switch between receptor desensitization and alternative signaling functions. This modification influences the balance between canonical and non canonical pathways initiated at the plasma membrane.

Beyond receptor internalization, Beta arrestin 1 acts as a scaffold for signaling cascades including MAPK, AKT, and NF kappa B pathways. Studies with Phospho-Beta Arrestin 1 (Ser412) antibody have shown that phosphorylation at serine 412 alters scaffold assembly, thereby regulating downstream signaling specificity. This makes it an essential modification for determining cellular outcomes in response to hormones, neurotransmitters, and growth factors.

Dysregulation of Beta arrestin 1 phosphorylation has been linked to disease. Research using Phospho-Beta Arrestin 1 (Ser412) antibody has revealed that altered phosphorylation patterns contribute to cardiovascular disease, neurological disorders, and cancer. In heart failure, aberrant phosphorylation disrupts receptor regulation, while in cancer, changes in Beta arrestin 1 signaling affect cell proliferation and metastasis. These observations underscore the importance of precise phosphorylation control in health and pathology.

Phospho-Beta Arrestin 1 (Ser412) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms phosphorylation status, immunohistochemistry highlights tissue specific activation patterns, and immunofluorescence demonstrates subcellular localization of phosphorylated arrestin in endosomes and receptor complexes. These experimental approaches make the antibody indispensable for signal transduction research.

By providing validated Phospho-Beta Arrestin 1 (Ser412) antibody reagents, NSJ Bioreagents supports research into receptor regulation, scaffold signaling, and disease mechanisms. Detection of phosphorylation at Ser412 provides precise insight into how Beta arrestin 1 controls G protein coupled receptor function.

UniProt: P49407

Pathways: Positive Regulation of Peptide Hormone Secretion, Nuclear Hormone Receptor Binding, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction