Recombinant PLCB3 antibody (pSer537)

Catalog No. ABIN7879757

This Rabbit Monoclonal antibody specifically detects PLCB3 in WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant PLCB3 antibody (pSer537) (ABIN7879757)

Target: PLCB3 (phospholipase C, beta 3 (Phosphatidylinositol-Specific) (PLCB3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PLCB3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 32P57

Product Details anti-PLCB3 Antibody

Binding Specificity: pSer537

Purpose: Phospho-PLCB3 (Ser537) Antibody / Phospholipase C beta 3

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-PLCB3 (S537) was used as the immunogen for the Phospho-PLCB3 (Ser537) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-PLCB3 (Ser537) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-PLCB3 (Ser537) antibody at -20oC.

Target Details for PLCB3

Target: PLCB3 (phospholipase C, beta 3 (Phosphatidylinositol-Specific) (PLCB3))

Alternative Name: PLCB3

Background: Phospho-PLCB3 (Ser537) antibody detects phospholipase C beta 3 (PLCB3) phosphorylated at serine 537, a modification that regulates enzyme activity and signaling. PLCB3 is a member of the phospholipase C family of enzymes, which hydrolyze phosphatidylinositol 4,5-bisphosphate (PIP2) to generate second messengers inositol trisphosphate (IP3) and diacylglycerol (DAG). These messengers mobilize intracellular calcium and activate protein kinase C, driving pathways involved in cell growth, differentiation, and immune responses.

Research using Phospho-PLCB3 (Ser537) antibody has shown that phosphorylation at this site enhances or modulates enzyme activity depending on cellular context. Kinases including PKC and CaMKII target Ser537, linking feedback regulation to receptor-mediated signaling. This modification influences G protein-coupled receptor (GPCR) and receptor tyrosine kinase pathways, where PLCB3 acts as a critical mediator of calcium signaling.

In immune cells, PLCB3 phosphorylation at Ser537 regulates lymphocyte activation, cytokine production, and inflammatory responses. Dysregulated PLCB3 activity contributes to autoimmune disease, chronic inflammation, and impaired host defense. In cardiovascular research, PLCB3 is implicated in vascular smooth muscle contraction, hypertension, and cardiac hypertrophy. Aberrant PLCB3 signaling has also been linked to tumorigenesis, where altered calcium signaling drives proliferation and survival.

Neurobiology studies show that PLCB3 contributes to synaptic plasticity and neurotransmitter release, with Ser537 phosphorylation fine-tuning neuronal calcium dynamics. This role connects PLCB3 to learning, memory, and neurological disorders. In endocrine systems, PLCB3 signaling regulates hormone secretion and metabolic processes.

Antibodies against phospho-PLCB3 (Ser537) are validated for western blot, immunohistochemistry, and immunofluorescence. These reagents allow detection of phosphorylation-dependent activation, supporting studies in signaling pathways and disease models.

NSJ Bioreagents supplies this Phospho-PLCB3 (Ser537) antibody for research into calcium signaling, immunity, and cancer biology.

UniProt: Q01970

Pathways: WNT Signaling, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, CXCR4-mediated Signaling Events, G-protein mediated Events