Recombinant ULK1 antibody (pSer556)

Catalog No. ABIN7879758

The Rabbit Monoclonal anti-ULK1 antibody is suitable to detect ULK1 in samples from Human and Mouse. It has been validated for WB, IF and ICC.

Quick Overview for Recombinant ULK1 antibody (pSer556) (ABIN7879758)

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ULK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 31U30

Product Details anti-ULK1 Antibody

Binding Specificity: pSer556

Purpose: Phospho-ULK1 (Ser556) Antibody / Unc-51 like autophagy activating kinase 1

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Phospho-ULK1 (S556) was used as the immunogen for the Phospho-ULK1 (Ser556) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-ULK1 (Ser556) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-ULK1 (Ser556) antibody at -20oC.

Target Details for ULK1

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Alternative Name: ULK1

Background: Phospho-ULK1 (Ser556) antibody detects Unc-51 like autophagy activating kinase 1 phosphorylated at serine 556, encoded by the ULK1 gene. ULK1 is a serine/threonine kinase that initiates autophagy in response to nutrient status and energy stress. Phosphorylation at Ser556 is an important regulatory event that integrates upstream signaling pathways to control autophagosome formation. Phospho-ULK1 (Ser556) antibody provides researchers with a highly specific reagent to study the regulation of autophagy and its role in physiology and disease.

ULK1 functions as the mammalian homolog of yeast Atg1, acting at the top of the autophagy hierarchy. Research using Phospho-ULK1 (Ser556) antibody has demonstrated that phosphorylation at serine 556 by AMP activated protein kinase promotes activation of ULK1 under conditions of energy depletion. This modification stimulates autophagy initiation by enabling the ULK1 complex to recruit downstream autophagy machinery. By contrast, mTORC1 signaling suppresses ULK1 through phosphorylation at other sites, highlighting the dynamic control of ULK1 activity by nutrient and energy signals.

Phospho-ULK1 (Ser556) antibody has been used to show that phosphorylation at this site acts as a molecular switch for autophagy induction. In starvation or low energy states, phosphorylation at Ser556 ensures cells adapt by recycling cytoplasmic contents through lysosomal degradation. This process provides metabolic substrates for survival and maintains homeostasis. Inhibiting phosphorylation at Ser556 impairs autophagy, underscoring its essential role in this pathway.

Dysregulation of ULK1 phosphorylation has been linked to human disease. Studies with Phospho-ULK1 (Ser556) antibody have revealed that impaired autophagy contributes to neurodegenerative diseases, metabolic disorders, and cancer. In cancer, altered ULK1 phosphorylation influences tumor cell survival under stress conditions, promoting growth in nutrient-poor environments. In neurodegeneration, reduced ULK1 activation leads to accumulation of toxic proteins. These findings emphasize the clinical importance of monitoring ULK1 phosphorylation states.

Phospho-ULK1 (Ser556) antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting reveals phosphorylation-dependent shifts in ULK1 activity, immunohistochemistry highlights tissue-specific patterns of autophagy activation, and immunofluorescence demonstrates dynamic localization of phosphorylated ULK1 at autophagosome initiation sites. These applications make Phospho-ULK1 (Ser556) antibody an indispensable reagent in autophagy research.

By supplying validated Phospho-ULK1 (Ser556) antibody reagents, NSJ Bioreagents supports research into energy sensing, autophagy regulation, and disease mechanisms. Detection of phosphorylation at Ser556 provides a precise marker of ULK1 activation and autophagy initiation.

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagy