ERN1 antibody (pSer724)

Catalog No. ABIN7879768

The Rabbit Monoclonal anti-ERN1 antibody (Clone EGH-5) (ABIN7879768) specifically detects ERN1 in WB. The antibody is reactive with Human samples.

Quick Overview for ERN1 antibody (pSer724) (ABIN7879768)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ERN1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: EGH-5

Product Details anti-ERN1 Antibody

Binding Specificity: pSer724

Purpose: Phospho-IRE1 Antibody (pS724)

Purification: Affinity purified

Immunogen: A synthetic peptide specific to human IRE1 / ERN1 (surrounding pS724) was used as the immunogen for the phospho-IRE1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the phospho-IRE1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in PBS with 0.02 % sodium azide, 50 % glycerol and 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the phospho-IRE1 antibody at -20oC.

Target Details for ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: IRE1

Background: IRE1 is a serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the ER luminal domain is maintained in its inactive monomeric state by binding to the ER chaperone HSPA5/BiP. Accumulation of misfolded protein in the ER causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. [UniProt]

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response