Recombinant MST1/MST2 (pThr180), (pThr183) antibody

Catalog No. ABIN7879784

This Rabbit Monoclonal antibody specifically detects MST1/MST2 in WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant MST1/MST2 (pThr180), (pThr183) antibody (ABIN7879784)

Target: MST1/MST2

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Clone: 32S47

Product Details

Binding Specificity: pThr180, pThr183

Purpose: Phospho-MST1/MST2 (Thr183/Thr180) Antibody

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-MST1/MST2 (T183 + T180) was used as the immunogen for the Phospho-MST1/MST2 (Thr183/Thr180) antibody .

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-MST1/MST2 (Thr183/Thr180) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-MST1/MST2 (Thr183/Thr180) antibody at -20oC.

Target Details

Target: MST1/MST2

Background: Phospho-MST1/MST2 (Thr183/Thr180) antibody detects the phosphorylated forms of MST1 (Thr183) and MST2 (Thr180), two core kinases in the Hippo signaling pathway. MST1 and MST2 are serine/threonine kinases belonging to the STE20 family and function as tumor suppressors by regulating cell proliferation, apoptosis, and organ size. Phosphorylation at these threonine residues is essential for their activation and downstream signaling through LATS kinases and transcriptional co-activators such as YAP and TAZ.

Research using Phospho-MST1/MST2 (Thr183/Thr180) antibody highlights their role in developmental biology and cancer. The Hippo pathway restricts cell growth, and loss of MST1/2 activity leads to YAP/TAZ hyperactivation, which promotes tumor initiation and metastasis. Conversely, phosphorylation-mediated activation of MST1/2 enhances apoptotic signaling and prevents uncontrolled proliferation. Mutations and dysregulation of this pathway have been implicated in hepatocellular carcinoma, breast cancer, colorectal cancer, and sarcomas.

Beyond oncology, MST1/2 activity influences immune system regulation and cardiovascular health. In T cells, MST1 regulates trafficking, adhesion, and immune synapse formation. Deficiency in MST1 has been linked to primary immunodeficiency disorders characterized by recurrent infections and defective lymphocyte migration. In cardiac biology, MST kinases contribute to heart development and stress responses, with phosphorylation regulating apoptosis in cardiomyocytes during ischemic injury.

Neurobiology research also benefits from Phospho-MST1/MST2 (Thr183/Thr180) antibody, as MST1 phosphorylation has been linked to neuronal apoptosis in models of stroke and neurodegenerative disease. Monitoring phosphorylation levels provides insights into mechanisms of neuronal injury and survival. In metabolic studies, Hippo pathway signaling intersects with insulin and mTOR pathways, suggesting MST1/2 phosphorylation affects energy balance and metabolism.

Antibodies specific for phospho-MST1/2 are validated for western blot, immunohistochemistry, and immunofluorescence. These reagents selectively detect the active, phosphorylated kinases, distinguishing them from total MST1/2. Clone-based antibodies provide reproducibility for tracking phosphorylation dynamics in diverse tissues and experimental systems.

NSJ Bioreagents offers this Phospho-MST1/MST2 (Thr183/Thr180) antibody to support research in Hippo signaling, cancer, immunity, and cardiovascular biology.

UniProt: Q13043