Recombinant CAMK2A antibody (pThr286)

Catalog No. ABIN7879791

The Rabbit Monoclonal anti-CAMK2A antibody is suitable to detect CAMK2A in samples from Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for Recombinant CAMK2A antibody (pThr286) (ABIN7879791)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Antibody Type: Recombinant Antibody

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CAMK2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 31C26

Product Details anti-CAMK2A Antibody

Binding Specificity: pThr286

Purpose: Phospho-CaMKII alpha (T286) Antibody / CAMK2A

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Phospho-CaMKII alpha (T286) was used as the immunogen for the Phospho-CaMKII alpha (T286) antibody. Due to the conserved nature of the epitope, the antibody will likely also detect Phospho-CaMKII beta (T287).

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-CaMKII alpha (T286) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-CaMKII alpha (T286) antibody at -20oC.

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: CaMKII alpha

Background: Phospho-CaMKII alpha (T286) antibody detects calcium/calmodulin-dependent protein kinase II alpha when phosphorylated at threonine 286. The protein is encoded by the CAMK2A gene and is commonly referred to as CaM kinase II alpha, CaMK-II subunit alpha, and calcium/calmodulin-dependent protein kinase type II alpha chain. CaMKII alpha is a serine/threonine kinase expressed abundantly in the brain, where it regulates synaptic plasticity, learning, and memory. Autophosphorylation at threonine 286 converts the kinase into a calcium/calmodulin-independent active form, a hallmark event in long-term potentiation and neuronal signaling.

Phospho-CaMKII alpha (T286) antibody is widely applied in neuroscience, synaptic biology, and signaling research. Detecting phosphorylation at this site provides a direct measure of CaMKII activation, linking cellular activity to memory formation. In neurons, Thr286 phosphorylation prolongs kinase activity beyond transient calcium spikes, enabling sustained synaptic strengthening. By targeting this phosphorylation event, researchers can explore how activity-dependent signaling translates into lasting changes in connectivity.

Applications for Phospho-CaMKII alpha (T286) antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylated CaMKII alpha isoforms in brain lysates, immunohistochemistry maps activation in hippocampus and cortex, and immunofluorescence reveals localization at dendritic spines. These methods provide detailed views of synaptic signaling in vitro and in vivo.

Phosphorylation at Thr286 has been extensively studied in relation to long-term potentiation, a cellular mechanism of memory storage. Abnormal regulation of CaMKII phosphorylation contributes to neurological disorders, including Alzheimer disease, schizophrenia, and epilepsy. By applying Phospho-CaMKII alpha (T286) antibody, scientists can investigate disease-related disruptions in synaptic plasticity.

Beyond neuroscience, CaMKII alpha also participates in cardiac signaling and metabolism. Phosphorylation at Thr286 has been detected in cardiac stress responses and metabolic regulation. The antibody therefore supports research into CaMKII biology across systems. NSJ Bioreagents offers Phospho-CaMKII alpha (T286) antibody with validated specificity, ensuring reliable results in neuronal and disease studies.

UniProt: Q9UQM7

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction