Recombinant PKC alpha antibody (pThr497)

Catalog No. ABIN7879798

The Rabbit Monoclonal anti-PKC alpha antibody (Clone 32P00) (ABIN7879798) specifically detects PKC alpha in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant PKC alpha antibody (pThr497) (ABIN7879798)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 32P00

Product Details anti-PKC alpha Antibody

Binding Specificity: pThr497

Purpose: Phospho-PKC alpha (Thr497) Antibody / Protein kinase C alpha

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-PKC alpha (T497) was used as the immunogen for the Phospho-PKC alpha (Thr497) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-PKC alpha (Thr497) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-PKC alpha (Thr497) antibody at -20oC.

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PKC alpha

Background: Phospho-PKC alpha Thr497 antibody recognizes protein kinase C alpha phosphorylated at threonine 497, an activation site essential for catalytic function. PKC alpha is a member of the conventional protein kinase C family that functions as a serine threonine kinase. It is activated by calcium, diacylglycerol, and phospholipids, and it regulates processes including cell proliferation, differentiation, apoptosis, and migration. Phosphorylation at Thr497 stabilizes the kinase in an active conformation and promotes downstream substrate phosphorylation.

Phospho-PKC alpha Thr497 antibody is widely applied in signaling and phosphorylation research. By distinguishing the phosphorylated active form from inactive PKC alpha, it enables precise monitoring of pathway activation. This is particularly relevant in studies of cancer, cardiovascular disease, and neuronal signaling where PKC alpha activity is tightly regulated.

The antibody is validated for use in western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho PKC alpha Thr497 antibody detects phosphorylated isoforms and allows quantification of activation under various stimuli. In immunohistochemistry, it highlights phosphorylation dependent localization within tissues, while immunofluorescence provides single cell resolution of active PKC alpha.

PKC alpha has been implicated in cancer progression, where dysregulated activation contributes to survival, proliferation, and metastasis. Monitoring Thr497 phosphorylation with phospho specific antibodies provides mechanistic insight into tumor biology and therapeutic targeting. In cardiovascular research, PKC alpha activity is linked to hypertrophy, contractility, and vascular remodeling. In the nervous system, PKC alpha influences synaptic plasticity and long term potentiation.

Phospho-PKC alpha Thr497 antibody offered by NSJ Bioreagents provides strong specificity for activated PKC alpha, supporting studies across oncology, cardiology, and neuroscience. It is a critical reagent for examining phosphorylation dependent signaling in health and disease.

UniProt: P17252

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling