Recombinant PBK antibody (pThr9)

Catalog No. ABIN7879809

The Rabbit Monoclonal anti-PBK antibody is suitable to detect PBK in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant PBK antibody (pThr9) (ABIN7879809)

Target: PBK (PDZ Binding Kinase (PBK))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PBK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 30P33

Product Details anti-PBK Antibody

Binding Specificity: pThr9

Purpose: Phospho-PBK (Thr9) Antibody / PDZ binding kinase

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-PBK/TOPK (Thr9) was used as the immunogen for the Phospho-PBK (Thr9) Antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-PBK (Thr9) Antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-PBK (Thr9) Antibody at -20oC.

Target Details for PBK

Target: PBK (PDZ Binding Kinase (PBK))

Alternative Name: PBK

Background: Phospho-PBK (Thr9) antibody detects PDZ binding kinase, also known as lymphokine activated killer T cell origin protein kinase (TOPK), when phosphorylated at threonine 9. PBK is encoded by the PBK gene and belongs to the MAP kinase kinase family. It is highly expressed in proliferating cells, especially in testis, placenta, and activated lymphocytes. Phosphorylation at Thr9 regulates PBK kinase activity, influencing cell cycle progression and mitotic events. This makes Phospho-PBK (Thr9) antibody a key tool for studying proliferative signaling and tumor biology.

Phospho-PBK (Thr9) antibody is widely applied in oncology, immunology, and cell cycle research. PBK activation supports G2/M transition by phosphorylating downstream substrates involved in spindle formation and chromosome segregation. It also participates in immune signaling pathways in T cells and NK cells. By detecting phosphorylation at Thr9, researchers can monitor PBK activation and its role in proliferation and immunity.

Applications for Phospho-PBK (Thr9) antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blot assays detect phosphorylated PBK isoforms, immunohistochemistry maps expression in tumors, and immunofluorescence reveals subcellular localization at centrosomes during mitosis. These complementary approaches provide detailed evaluation of PBK biology.

Dysregulated PBK activity is implicated in multiple cancers, including lung, breast, and hematologic malignancies. Elevated Thr9 phosphorylation correlates with tumor grade and poor prognosis, reflecting hyperactive proliferation. In immune biology, PBK phosphorylation regulates lymphocyte activation, linking cell cycle control to host defense. By applying Phospho-PBK (Thr9) antibody, scientists can study these dual roles in cancer and immunity.

Therapeutic research has targeted PBK as a potential kinase for inhibition. Small molecule inhibitors reduce proliferation and sensitize tumor cells to chemotherapy. Monitoring Thr9 phosphorylation provides a pharmacodynamic readout of drug activity. Phospho-PBK (Thr9) antibody from NSJ Bioreagents offers strong specificity for this site specific modification, supporting mechanistic and translational research into kinase biology.

UniProt: Q96KB5