Recombinant Caveolin-1 antibody (pTyr14)

Catalog No. ABIN7879827

The Rabbit Monoclonal anti-Caveolin-1 antibody is suitable to detect Caveolin-1 in samples from Human. It has been validated for WB.

Quick Overview for Recombinant Caveolin-1 antibody (pTyr14) (ABIN7879827)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 32C49

Product Details anti-Caveolin-1 Antibody

Binding Specificity: pTyr14

Purpose: Phospho-Caveolin 1 (Tyr14) Antibody / CAV1

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Phospho-Caveolin-1 (Y14) was used as the immunogen for the Phospho-Caveolin 1 (Tyr14) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-Caveolin 1 (Tyr14) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-Caveolin 1 (Tyr14) antibody at -20oC.

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: Caveolin 1

Background: Phospho-Caveolin 1 (Tyr14) antibody detects Caveolin 1 phosphorylated at tyrosine 14, encoded by the CAV1 gene. Caveolin 1 is an integral membrane protein and principal component of caveolae, small invaginations in the plasma membrane involved in endocytosis, cholesterol homeostasis, and signal transduction. Phosphorylation at tyrosine 14 is a key regulatory modification that alters Caveolin 1 function and interactions with signaling molecules. Phospho-Caveolin 1 (Tyr14) antibody provides researchers with a powerful tool for investigating membrane dynamics and signaling pathways regulated by caveolae.

Caveolin 1 acts as a scaffolding protein that organizes signaling complexes at the plasma membrane. Research using Phospho-Caveolin 1 (Tyr14) antibody has demonstrated that phosphorylation at tyrosine 14 is mediated primarily by Src family kinases in response to growth factors, mechanical stress, or cellular adhesion events. This modification induces conformational changes that modulate protein interactions and influence downstream signaling. By altering binding partners, phosphorylation at Tyr14 affects processes such as focal adhesion turnover, cell migration, and proliferation.

Studies with Phospho-Caveolin 1 (Tyr14) antibody have shown that this modification regulates integrin signaling and cytoskeletal dynamics. Phosphorylated Caveolin 1 promotes disassembly of focal adhesions, allowing cells to migrate during wound healing, angiogenesis, and cancer metastasis. It also participates in mechanotransduction, enabling cells to sense and respond to changes in extracellular matrix stiffness or fluid shear stress. These findings highlight the versatility of Caveolin 1 phosphorylation as a regulatory switch in cell biology.

Dysregulation of Caveolin 1 phosphorylation has been linked to multiple diseases. Research using Phospho-Caveolin 1 (Tyr14) antibody has revealed altered phosphorylation in cancer, where excessive modification promotes tumor cell invasion and metastasis. In cardiovascular disease, abnormal Caveolin 1 phosphorylation disrupts endothelial function and contributes to vascular pathology. In pulmonary fibrosis, changes in Caveolin 1 phosphorylation status affect fibroblast activation and extracellular matrix remodeling. These diverse roles emphasize its broad clinical relevance.

Phospho-Caveolin 1 (Tyr14) antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes phosphorylated Caveolin 1 from its unmodified form, immunohistochemistry identifies activated signaling domains in tissues, and immunofluorescence visualizes phosphorylated Caveolin 1 within caveolae at the plasma membrane. These applications make the antibody indispensable for cell signaling and membrane biology research.

By providing validated Phospho-Caveolin 1 (Tyr14) antibody reagents, NSJ Bioreagents supports studies into membrane signaling, cytoskeletal regulation, and disease processes. Detection of Caveolin 1 phosphorylation at tyrosine 14 offers a highly specific marker of caveolae mediated signaling activity.

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals