Recombinant CrkL antibody (pTyr207)

Catalog No. ABIN7879829

This Rabbit Monoclonal antibody specifically detects CrkL in WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant CrkL antibody (pTyr207) (ABIN7879829)

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CrkL antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 32C08

Product Details anti-CrkL Antibody

Binding Specificity: pTyr207

Purpose: Phospho-CrkL (Tyr207) Antibody / Crk like protein

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Phospho-CrkL (Y207) was used as the immunogen for the Phospho-CrkL (Tyr207) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-CrkL (Tyr207) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-CrkL (Tyr207) antibody at -20oC.

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: CrkL

Background: Phospho-CrkL (Tyr207) antibody detects the phosphorylated form of Crk like protein, encoded by the CRKL gene. Crk like is an adaptor protein that mediates intracellular signaling downstream of multiple tyrosine kinases, including BCR ABL, EGFR, and PDGFR. It contains SH2 and SH3 domains that allow it to bind phosphotyrosine residues and recruit effector proteins, thereby linking receptor activation to downstream pathways. Phosphorylation at tyrosine 207 is a regulatory modification that influences Crk like protein interactions and signaling capacity. Phospho-CrkL (Tyr207) antibody provides researchers with a reliable reagent to study tyrosine kinase signaling in normal and malignant cells.

Crk like phosphorylation at tyrosine 207 is mediated by kinases such as ABL and has been shown to modulate protein conformation and adaptor activity. Research using Phospho-CrkL (Tyr207) antibody has demonstrated that phosphorylation reduces binding to certain partners while promoting interactions with negative regulators, thus acting as a feedback mechanism to control signaling intensity. This fine tuning ensures that pathways such as Ras, Rac, and PI3K are activated appropriately in response to growth factor stimulation or stress signals.

Aberrant phosphorylation of Crk like at tyrosine 207 has been strongly associated with leukemia, particularly chronic myeloid leukemia, where BCR ABL fusion kinase constitutively phosphorylates this residue. Phospho-CrkL (Tyr207) antibody has therefore become a surrogate marker for BCR ABL activity in both research and clinical contexts. Monitoring Crk like phosphorylation provides a sensitive measure of kinase inhibition in patients treated with tyrosine kinase inhibitors, making it a valuable biomarker for therapeutic response. Beyond leukemia, altered phosphorylation has been observed in solid tumors, linking Crk like to diverse oncogenic pathways.

Phospho-CrkL (Tyr207) antibody is used in western blotting, immunoprecipitation, and flow cytometry. Western blotting provides detection of phosphorylated protein as an indicator of kinase activity. Immunoprecipitation with this antibody isolates Crk like protein complexes, allowing characterization of binding partners under different phosphorylation states. Flow cytometry applications extend its use to patient samples, where phosphorylation levels can be measured at the single cell level. These diverse applications demonstrate the versatility of Phospho-CrkL (Tyr207) antibody in both laboratory research and translational medicine.

By providing Phospho-CrkL (Tyr207) antibody, NSJ Bioreagents supports research into tyrosine kinase signaling, cancer biology, and therapeutic monitoring. Detection of Crk like phosphorylation at tyrosine 207 offers a powerful means of understanding adaptor protein regulation and its impact on cellular behavior.

UniProt: P46109

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor