Recombinant Actin alpha (ACTAlpha) antibody

Catalog No. ABIN7879867

The Rabbit Monoclonal anti-Actin alpha (ACTAlpha) antibody (Clone CAD-1) (ABIN7879867) specifically detects Actin alpha (ACTAlpha) in WB, IHC (p) and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant Actin alpha (ACTAlpha) antibody (ABIN7879867)

Target: Actin alpha (ACTAlpha)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: CAD-1

Product Details

Purpose: Alpha Actin Antibody / ACTA2 / Smooth Muscle Actin

Purification: Affinity purified

Immunogen: A synthetic peptide specific to human ACTA2 protein was used as the immunogen for the Alpha Actin antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Alpha Actin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in PBS with 0.02 % sodium azide, 50 % glycerol and 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Alpha Actin antibody at -20oC.

Target Details

Target: Actin alpha (ACTAlpha)

Alternative Name: Alpha Actin

Background: Alpha Actin antibody is a key reagent for studying cytoskeletal dynamics, smooth muscle physiology, and tissue remodeling. The encoded protein, ACTA2 (actin alpha 2), is a smooth muscle specific isoform of actin that contributes to contraction, structure, and motility of vascular and visceral smooth muscle cells. As a fundamental component of the cytoskeleton, ACTA2 polymerizes into filaments that provide mechanical strength and coordinate intracellular signaling.

Alpha actin is highly expressed in the smooth muscle layers of blood vessels, the respiratory tract, the gastrointestinal system, and reproductive organs. It also serves as a well established marker for myofibroblasts, which emerge during wound healing and fibrotic disease. Upregulation of ACTA2 in fibroblasts indicates their transition into contractile myofibroblasts, a process central to tissue repair and pathologic fibrosis. Detection with Alpha Actin antibody allows researchers to track these changes in both normal physiology and disease progression.

In cardiovascular research, ACTA2 is examined as a marker of vascular smooth muscle cells and their response to injury or remodeling. Phenotypic switching of smooth muscle cells, from a contractile to a synthetic state, is associated with vascular disease such as atherosclerosis. In liver, kidney, and lung fibrosis, ACTA2 positive myofibroblasts accumulate and contribute to excessive extracellular matrix deposition. Because of this, the Alpha Actin antibody is routinely applied in histological studies of fibrotic disorders.

At the molecular level, ACTA2 shares high sequence similarity with other actin isoforms but contains unique features that confer smooth muscle specific function. It interacts with actin binding proteins to regulate filament turnover, contractility, and cell migration. The strong conservation of actin family proteins ensures that Alpha Actin antibody provides reliable detection across species in research models.

The Alpha Actin antibody is widely used in immunohistochemistry, immunofluorescence, western blotting, and flow cytometry. These applications enable localization and quantification of ACTA2 in tissue samples and cultured cells. For researchers focused on vascular biology, tissue fibrosis, or cytoskeletal organization, the Alpha Actin antibody is a specific and dependable detection tool. NSJ Bioreagents offers validated antibodies to ensure reproducibility and accuracy in molecular and cellular studies.

UniProt: P62736