Recombinant BCLAF1 antibody

Catalog No. ABIN7880060

This Rabbit Monoclonal antibody specifically detects BCLAF1 in WB, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant BCLAF1 antibody (ABIN7880060)

Target: BCLAF1 (BCL2-Associated Transcription Factor 1 (BCLAF1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BCLAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 32B81

Product Details anti-BCLAF1 Antibody

Purpose: BCLAF1 Antibody / BCL2-associated transcription factor 1 / BTF

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human BTF was used as the immunogen for the BCLAF1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the BCLAF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the BCLAF1 antibody at -20oC.

Target Details for BCLAF1

Target: BCLAF1 (BCL2-Associated Transcription Factor 1 (BCLAF1))

Alternative Name: BCLAF1

Background: BCLAF1 antibody detects BCL2-associated transcription factor 1, encoded by the BCLAF1 gene. BCL2-associated transcription factor 1 is a nuclear protein originally identified through its binding to anti-apoptotic members of the Bcl-2 family. BCLAF1 antibody provides researchers with a highly specific reagent to study apoptosis, transcriptional regulation, and cellular stress responses.

BCL2-associated transcription factor 1 contains multiple functional domains that enable it to interact with transcriptional regulators, splicing machinery, and apoptotic signaling proteins. Research using BCLAF1 antibody has shown that it can act as a transcriptional repressor, influencing gene networks associated with cell death and survival. Its ability to interact with Bcl-2 family proteins positions it at the intersection of transcription and apoptosis regulation.

Studies with BCLAF1 antibody have demonstrated that the protein also functions in RNA processing. BCL2-associated transcription factor 1 associates with splicing factors and contributes to pre-mRNA processing. This activity provides an additional layer of gene expression control, ensuring that transcription and splicing are coordinated in response to cellular needs.

Beyond transcription and splicing, BCLAF1 influences DNA damage responses. Research using BCLAF1 antibody has revealed that the protein participates in DNA repair pathways and helps maintain genomic stability. Its expression is upregulated in response to genotoxic stress, and it contributes to apoptosis when DNA damage is severe. This dual role in repair and apoptosis underscores its importance in genome maintenance.

Dysregulation of BCLAF1 has been linked to cancer and immune disorders. Overexpression can enhance apoptosis and impair cell survival, while reduced expression contributes to resistance to cell death. Studies with BCLAF1 antibody have also associated mutations and altered expression with autoimmune diseases. These findings demonstrate that balanced regulation of BCL2-associated transcription factor 1 is essential for normal physiology.

BCLAF1 antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects full-length protein and isoforms, immunohistochemistry identifies nuclear expression patterns in tissues, and immunofluorescence reveals its nuclear localization and distribution within subnuclear domains. These applications make BCLAF1 antibody valuable for research into transcription, apoptosis, and DNA repair.

By supplying validated BCLAF1 antibody reagents, NSJ Bioreagents supports research into apoptosis regulation, nuclear function, and disease biology. Detection of BCL2-associated transcription factor 1 provides insight into how cells integrate transcriptional control with survival and death signals.

UniProt: Q9NYF8

Pathways: Positive Regulation of Response to DNA Damage Stimulus