Recombinant Biotin antibody

Catalog No. ABIN7880080

The Rabbit Monoclonal anti-Biotin antibody (Clone BTN-2032R) (ABIN7880080) specifically detects Biotin in IHC (p).

Quick Overview for Recombinant Biotin antibody (ABIN7880080)

Target: Biotin

Antibody Type: Recombinant Antibody

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: BTN-2032R

Product Details anti-Biotin Antibody

Purpose: Recombinant Biotin Antibody / Rabbit Monoclonal (azide and preservative free)

Purification: Protein A affinity chromatography

Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this recombinant Biotin antibody.

Isotype: IgG, kappa

Application Details

Application Notes: The optimal dilution of the recombinant Biotin antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Biotin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.