BrdU antibody

Catalog No. ABIN7880101

The Mouse Monoclonal anti-BrdU antibody (Clone SPM537) (ABIN7880101) specifically detects BrdU in IHC (p).

Quick Overview for BrdU antibody (ABIN7880101)

Target: BrdU (Bromodeoxyuridine (BrdU))

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM537

Product Details anti-BrdU Antibody

Purpose: Anti-BrdU Antibody

Purification: Protein G affinity chromatography

Immunogen: Bromodeoxyuridine was used as the immunogen for this anti-BrdU antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the anti-BrdU antibody for each application should be determined by the researcher.

1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with Trypsin at 1 mg/mL PBS, 10 min at 37oC.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU

Target Type: Chemical

Background: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.