CD3D antibody

Catalog No. ABIN7880290

The Rabbit Monoclonal anti-CD3D antibody (Clone IDE-3) (ABIN7880290) specifically detects CD3D in WB, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CD3D antibody (ABIN7880290)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD3D antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IDE-3

Product Details anti-CD3D Antibody

Purpose: CD3D Antibody / CD3 delta

Purification: Affinity purified

Immunogen: A synthetic peptide specific to human CD3D was used as the immunogen for the CD3D antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CD3D antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in PBS with 0.02 % sodium azide, 50 % glycerol and 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the CD3D antibody at -20oC.

Target Details for CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Alternative Name: CD3D

Background: CD3D antibody recognizes CD3 delta subunit, a type I transmembrane protein encoded by the CD3D gene and commonly referred to as CD3 delta chain. CD3 delta is an essential component of the T cell receptor CD3 complex, which mediates antigen recognition and intracellular signaling in T lymphocytes. As part of the multimeric TCR-CD3 signaling complex, CD3 delta contributes to receptor assembly, membrane expression, and signal transduction required for adaptive immune responses. CD3D antibody supports research applications focused on T cell biology, immune signaling pathways, and lymphoid tissue characterization.

CD3 delta associates with CD3 gamma, CD3 epsilon, and CD3 zeta chains to form the invariant signaling module of the T cell receptor. Upon antigen engagement, immunoreceptor tyrosine-based activation motifs within the cytoplasmic domains of CD3 subunits undergo phosphorylation, triggering downstream signaling cascades that regulate T cell activation, proliferation, and cytokine production. CD3 delta localizes primarily to the plasma membrane of mature T cells, where it stabilizes receptor expression and contributes to immune synapse formation and signal amplification.

In normal human tissues, CD3 delta expression is restricted to T lymphocytes within thymus, lymph node, spleen, tonsil, and peripheral blood. Within lymphoid organs, CD3 staining highlights T cell-rich areas such as the paracortex of lymph nodes and interfollicular regions of tonsil, while B cell follicles and non-lymphoid stromal compartments remain largely negative. This lineage-specific distribution makes CD3D antibody a valuable marker for identifying T cell populations in research settings. Alterations in CD3 complex expression are relevant in studies of T cell immunodeficiencies and T cell leukemias or lymphomas, where receptor composition and signaling capacity may be disrupted.

Clone IDE-3 is a rabbit monoclonal antibody generated to recognize CD3 delta with defined specificity. CD3D antibody can be used to investigate T cell receptor signaling mechanisms, lymphoid architecture, and adaptive immune responses in experimental models. Its defined target recognition and membrane-associated staining profile support studies of T cell distribution and immune system function in normal and disease-associated tissues.

UniProt: P04234

Pathways: TCR Signaling, CXCR4-mediated Signaling Events