Recombinant CRABP2 antibody

Catalog No. ABIN7880384

This Rabbit Monoclonal antibody specifically detects CRABP2 in WB, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant CRABP2 antibody (ABIN7880384)

Target: CRABP2 (Cellular Retinoic Acid Binding Protein 2 (CRABP2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CRABP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 30C81

Product Details anti-CRABP2 Antibody

Purpose: CRABP2 Antibody / Cellular retinoic acid-binding protein 2

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human CRABP2 was used as the immunogen for the CRABP2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CRABP2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the CRABP2 antibody at -20oC.

Target Details for CRABP2

Target: CRABP2 (Cellular Retinoic Acid Binding Protein 2 (CRABP2))

Alternative Name: CRABP2

Background: CRABP2 antibody detects cellular retinoic acid-binding protein 2, encoded by the CRABP2 gene. Alternate names include CRABP-II, cellular retinoic acid-binding protein type II, and RA-binding protein 2. CRABP2 is a cytoplasmic protein that binds retinoic acid with high affinity, delivering it to the nucleus to interact with retinoic acid receptors (RARs). This shuttling mechanism ensures efficient retinoic acid signaling, which regulates cell differentiation, growth, and development. Structurally, CRABP2 belongs to the fatty acid-binding protein family, characterized by a beta-barrel fold that binds hydrophobic ligands.

CRABP2 antibody is widely applied in developmental biology, cancer research, and dermatology. Retinoic acid is essential for embryonic patterning, limb development, and organogenesis, and CRABP2 ensures delivery of ligand to nuclear receptors. In skin, CRABP2 regulates keratinocyte differentiation and retinoid responses, linking it to dermatological therapies. In cancer biology, CRABP2 expression modulates sensitivity to retinoids, influencing proliferation and apoptosis.

Applications of CRABP2 antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blotting detects CRABP2 protein in cell and tissue lysates, immunohistochemistry maps expression in embryonic and tumor tissues, and immunofluorescence highlights cytoplasmic and nuclear localization. These methods support research into retinoid signaling in diverse contexts.

Dysregulation of CRABP2 is linked to developmental abnormalities, cancer progression, and skin disease. Overexpression promotes retinoid sensitivity in some cancers, while downregulation confers resistance. By applying CRABP2 antibody, researchers can study how retinoid signaling contributes to disease and therapy response.

CRABP2 interacts with nuclear RARs, cytoplasmic enzymes, and signaling molecules. This positions it as a central mediator of vitamin A biology. NSJ Bioreagents provides CRABP2 antibody with validated specificity, supporting accurate detection in studies of development, cancer, and dermatology.

UniProt: P29373