Recombinant Nesprin3 antibody

Catalog No. ABIN7880435

This Rabbit Monoclonal antibody specifically detects Nesprin3 in WB. It exhibits reactivity toward Human.

Quick Overview for Recombinant Nesprin3 antibody (ABIN7880435)

Target: Nesprin3 (C14orf49) (Chromosome 14 Open Reading Frame 49 (C14orf49))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nesprin3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 30S37

Product Details anti-Nesprin3 Antibody

Purpose: SYNE3 Antibody / Nesprin 3

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Nesprin3 was used as the immunogen for the SYNE3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SYNE3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the SYNE3 antibody at -20oC.

Target Details for Nesprin3

Target: Nesprin3 (C14orf49) (Chromosome 14 Open Reading Frame 49 (C14orf49))

Alternative Name: SYNE3

Background: SYNE3 antibody detects Nesprin 3, encoded by the SYNE3 gene. This protein belongs to the spectrin repeat containing nuclear envelope protein family and is a component of the LINC complex, which connects the nuclear envelope to the cytoskeleton. Unlike other family members that link directly to actin filaments, Nesprin 3 connects to intermediate filaments through partners such as plectin, providing a unique bridge between the nucleus and cytoskeletal structures. Researchers use SYNE3 antibody to investigate nuclear positioning, mechanotransduction, and the maintenance of structural integrity across diverse cell types.

The LINC complex serves as a conduit for mechanical signals transmitted between the cytoplasm and the nucleus. By anchoring the nuclear envelope to cytoskeletal elements, Nesprin3 helps stabilize nuclear shape and position during migration, mitosis, and differentiation. When detected with SYNE3 antibody, changes in its expression or distribution can be correlated with altered mechanosensing and transcriptional regulation. These processes are essential for proper tissue development and for cellular responses to physical stress, making the study of SYNE3 protein highly relevant to mechanobiology.

Dysregulation of nuclear envelope proteins contributes to a variety of diseases, including muscular dystrophies, cardiomyopathies, and neurological disorders. Although more attention has historically focused on Nesprin1 and Nesprin2, studies suggest that Nesprin 3 also participates in these pathways by weakening nuclear anchorage when perturbed. SYNE3 antibody enables detection of protein alterations in both healthy and diseased tissue, providing a means to assess its contribution to pathogenesis. Evidence has also linked SYNE3 to cancer progression, where nuclear deformability affects metastatic cell migration through dense tissue matrices.

SYNE3 antibody is widely used in western blotting, immunofluorescence, and immunohistochemistry. In western blotting, it detects protein levels across tissue lysates, while immunofluorescence confirms its nuclear envelope localization in cultured cells. Immunohistochemistry highlights expression patterns in tissues such as muscle, skin, and epithelium, reflecting its role in nuclear architecture. Functional studies using SYNE3 antibody in knockdown and rescue experiments have demonstrated how loss of the protein disrupts nuclear positioning and cell motility, reinforcing its role in structural and signaling networks.

By providing validated SYNE3 antibody reagents, NSJ Bioreagents supports research into nuclear envelope biology, cytoskeletal connections, and disease mechanisms. Accurate detection of this nuclear envelope protein allows scientists to investigate how mechanical forces shape gene expression, development, and pathological states.

UniProt: Q6ZMZ3

Pathways: Maintenance of Protein Location