Recombinant AER61 antibody

Catalog No. ABIN7880438

This Rabbit Monoclonal antibody specifically detects AER61 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant AER61 antibody (ABIN7880438)

Target: AER61 (C3orf64) (Chromosome 3 Open Reading Frame 64 (C3orf64))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This AER61 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 30E02

Product Details anti-AER61 Antibody

Purpose: AER61 Antibody / EOGT

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human AER61 was used as the immunogen for the EOGT antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the EOGT antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the EOGT antibody at -20oC.

Target Details for AER61

Target: AER61 (C3orf64) (Chromosome 3 Open Reading Frame 64 (C3orf64))

Alternative Name: AER61

Background: EOGT antibody detects EGF domain specific O linked N acetylglucosamine transferase, an enzyme encoded by the EOGT gene. EOGT catalyzes the addition of O GlcNAc moieties to EGF like domains of secreted and membrane proteins, modifying signaling molecules and receptors. This post translational modification regulates ligand receptor interactions and signaling pathways critical for development and homeostasis.

EOGT antibody is widely used in studies of glycosylation, signaling, and developmental biology. By detecting EOGT, researchers can explore how O GlcNAc modification influences Notch signaling, growth factor binding, and tissue morphogenesis. Mutations in EOGT are associated with Adams Oliver syndrome, a developmental disorder characterized by limb and scalp defects, underscoring its biological importance.

In western blot assays, EOGT antibody detects protein bands corresponding to the enzyme in tissue and cell extracts. Immunohistochemistry maps expression in developing tissues, while immunofluorescence reveals subcellular localization consistent with endoplasmic reticulum and Golgi compartments. These methods support detailed analysis of glycosyltransferase biology.

EOGT mediated O GlcNAc modification contributes to signaling regulation in vascular and neural development. Dysregulation of this pathway has implications in congenital disorders, cancer, and immune regulation. By applying EOGT antibody, scientists can investigate how defects in protein glycosylation contribute to human disease and identify therapeutic strategies that target glycosylation pathways.

EOGT antibody from NSJ Bioreagents provides strong specificity for studying glycosylation and developmental biology. Its performance across multiple assays makes it a valuable reagent for exploring the role of O GlcNAc transferases in health and disease.

UniProt: Q5NDL2