Recombinant COBRA1 antibody

Catalog No. ABIN7880464

The Rabbit Monoclonal anti-COBRA1 antibody (Clone 32C80) (ABIN7880464) specifically detects COBRA1 in WB, IHC, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for Recombinant COBRA1 antibody (ABIN7880464)

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This COBRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 32C80

Product Details anti-COBRA1 Antibody

Purpose: NELFB Antibody / Negative elongation factor B / COBRA1

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human NELFB was used as the immunogen for the NELFB antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NELFB antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the NELFB antibody at -20oC.

Target Details for COBRA1

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Alternative Name: NELFB

Background: NELFB antibody targets negative elongation factor B, also known as COBRA1, a transcriptional regulator involved in RNA polymerase II pausing. NELFB functions as part of the NELF complex, which includes NELFA, NELFC/D, and NELFE subunits. This complex stabilizes paused RNA polymerase II at promoter-proximal regions, ensuring proper regulation of transcriptional elongation. By fine-tuning transcription, NELFB contributes to developmental programs and cellular stress responses.

Studies with NELFB antibody have implicated the protein in cancer biology and hormone signaling. COBRA1 was first identified as a BRCA1-associated factor, and disruption of NELFB function has been linked to breast cancer progression and altered estrogen receptor activity. Beyond cancer, NELFB also regulates genes involved in embryonic development and immune responses, making it a versatile factor for gene expression studies.

Validated antibody clones for NELFB detection work in assays such as western blot, chromatin immunoprecipitation, and immunohistochemistry. These reagents enable researchers to study promoter-proximal pausing, transcriptional control, and protein-protein interactions within the NELF complex. Clone validation ensures specificity for NELFB rather than closely related transcriptional regulators.

NSJ Bioreagents supplies this NELFB antibody for studies in transcription regulation, cancer pathways, and developmental biology. Alternate names include negative elongation factor B antibody, COBRA1 antibody, and cofactor of BRCA1 antibody.

UniProt: Q8WX92