G-CSF antibody

Catalog No. ABIN7880489

This Mouse Monoclonal antibody specifically detects G-CSF in IHC (p). It exhibits reactivity toward Human.

Quick Overview for G-CSF antibody (ABIN7880489)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This G-CSF antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM468

Product Details anti-G-CSF Antibody

Purpose: GCSF Antibody

Purification: Protein G affinity chromatography

Immunogen: Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes was used as the immunogen for the GCSF antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the GCSF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the GCSF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: GCSF

Background: This mAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSFs, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF s share 73 % sequence identity at the amino acid level.

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization