Recombinant DAZAP1 antibody

Catalog No. ABIN7880605

The Rabbit Monoclonal anti-DAZAP1 antibody (Clone 29D87) (ABIN7880605) specifically detects DAZAP1 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant DAZAP1 antibody (ABIN7880605)

Target: DAZAP1 (DAZ Associated Protein 1 (DAZAP1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This DAZAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 29D87

Product Details anti-DAZAP1 Antibody

Purpose: DAZAP1 Antibody / DAZ associated protein 1

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human DAZAP1 was used as the immunogen for the DAZAP1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the DAZAP1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the DAZAP1 antibody at -20oC.

Target Details for DAZAP1

Target: DAZAP1 (DAZ Associated Protein 1 (DAZAP1))

Alternative Name: DAZAP1

Background: DAZAP1 antibody detects DAZ associated protein 1, encoded by the DAZAP1 gene. This protein is an RNA binding factor involved in mRNA processing, alternative splicing, and germ cell development. DAZAP1 antibody provides researchers with a tool to explore reproductive biology, RNA metabolism, and gene regulation. Originally identified as a binding partner of Deleted in Azoospermia (DAZ), DAZAP1 is important in spermatogenesis and fertility.

DAZ associated protein 1 contains RNA recognition motifs that allow it to bind specific transcripts and influence splicing. Research using DAZAP1 antibody has demonstrated that it regulates pre-mRNA splicing of genes required for germ cell maturation. Its role in testis development makes it critical for fertility, and disruption of DAZAP1 function can impair spermatogenesis. Beyond reproduction, DAZAP1 is expressed in somatic tissues where it contributes to general RNA regulation.

DAZAP1 interacts with a variety of splicing factors and RNA processing proteins. Studies with DAZAP1 antibody have shown that it modulates exon inclusion or exclusion in target transcripts, thereby shaping tissue-specific gene expression. Alterations in DAZAP1 expression influence cell differentiation and development, suggesting a broader biological impact beyond germline function.

In disease contexts, DAZAP1 has been linked to cancer and developmental abnormalities. Research with DAZAP1 antibody has revealed that altered expression affects cell cycle regulation and apoptosis. Its misregulation in tumors suggests a potential role in oncogenesis, possibly through changes in RNA splicing that promote malignant phenotypes. These findings highlight DAZAP1 as both a fertility factor and a regulator of cell fate.

DAZAP1 antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein size and isoforms, immunohistochemistry demonstrates expression in testis and other tissues, and immunofluorescence shows nuclear localization with splicing speckles. These methods make DAZAP1 antibody versatile in RNA biology research.

By providing validated DAZAP1 antibody reagents, NSJ Bioreagents supports research into RNA processing, reproduction, and cancer biology. Detection of DAZ associated protein 1 enables researchers to examine how RNA binding proteins regulate fertility and somatic cell function.

UniProt: Q96EP5