Recombinant ERP27 antibody

Catalog No. ABIN7880685

The Rabbit Monoclonal anti-ERP27 antibody is suitable to detect ERP27 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ICC and IF.

Quick Overview for Recombinant ERP27 antibody (ABIN7880685)

Target: ERP27 (Endoplasmic Reticulum Protein 27 (ERP27))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ERP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 32E24

Product Details anti-ERP27 Antibody

Purpose: ERP27 Antibody / Endoplasmic reticulum resident protein 27 / TXNDC13

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human ERP27 was used as the immunogen for the ERP27 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ERP27 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the ERP27 antibody at -20oC.

Target Details for ERP27

Target: ERP27 (Endoplasmic Reticulum Protein 27 (ERP27))

Alternative Name: ERP27

Background: ERP27 antibody detects Endoplasmic reticulum resident protein 27, also called TXNDC13, encoded by the ERP27 gene. This protein belongs to the protein disulfide isomerase (PDI) family but is unique because it lacks catalytic cysteine residues required for disulfide bond formation. Instead, ERP27 functions as a non catalytic PDI family member that likely regulates protein folding through binding to unfolded substrates. ERP27 antibody provides a useful tool for studying protein folding, endoplasmic reticulum stress responses, and secretory pathway homeostasis.

Endoplasmic reticulum resident protein 27 is localized to the lumen of the endoplasmic reticulum, where it interacts with nascent polypeptides and folding intermediates. Although it lacks enzymatic activity, ERP27 binds to unfolded proteins, potentially presenting them to catalytic PDIs for isomerization. Research using ERP27 antibody has demonstrated that it contributes to quality control of protein folding, ensuring only properly folded proteins proceed through the secretory pathway. By acting as a chaperone like molecule, ERP27 complements enzymatic PDIs in maintaining proteostasis.

ERP27 is upregulated during conditions of endoplasmic reticulum stress, such as accumulation of unfolded proteins or perturbations in calcium homeostasis. Studies with ERP27 antibody have shown that its expression is regulated by the unfolded protein response, linking it to pathways that restore folding capacity and reduce stress. Dysregulation of ERP27 may contribute to diseases characterized by endoplasmic reticulum stress, including diabetes, neurodegeneration, and cancer. Understanding ERP27 expression and function helps clarify how cells cope with misfolded proteins and maintain secretory pathway fidelity.

In cancer, altered expression of endoplasmic reticulum chaperones influences tumor survival and progression. Research with ERP27 antibody has indicated that increased expression may provide tumor cells with resilience against proteotoxic stress, while reduced levels may impair secretory function. Its role in regulating folding efficiency positions ERP27 as an emerging factor in tumor biology and therapeutic resistance. Beyond cancer, ERP27 contributes to cellular stress adaptation across a wide range of tissues, including secretory cells of the pancreas and liver.

ERP27 antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting demonstrates induction under stress conditions, immunohistochemistry shows distribution in secretory tissues, and immunofluorescence confirms localization to the endoplasmic reticulum. These applications make ERP27 antibody valuable for research into protein folding, stress responses, and secretory pathway regulation.

By providing validated ERP27 antibody reagents, NSJ Bioreagents supports studies into protein folding, quality control, and disease. Detection of Endoplasmic reticulum resident protein 27 allows researchers to investigate how chaperone like proteins regulate proteostasis and contribute to pathology.

UniProt: Q96DN0