Fc gamma RII (CD32) antibody

Catalog No. ABIN7880777

This Mouse Monoclonal antibody specifically detects Fc gamma RII in FACS. It exhibits reactivity toward Human.

Quick Overview for Fc gamma RII (CD32) antibody (ABIN7880777)

Target: Fc gamma RII (CD32)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS)

Grade: Carrier-free

Clone: FCGR2A-479

Product Details

Purpose: CD32 Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the CD32 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the CD32 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: CD32

Background: This CD32 antibody binds most strongly to the IIa isoforms of CD32 (FcRII), a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

UniProt: P12318