Recombinant GRSF1 antibody

Catalog No. ABIN7880840

This Rabbit Monoclonal antibody specifically detects GRSF1 in WB, IF, IHC, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant GRSF1 antibody (ABIN7880840)

Target: GRSF1 (G-Rich RNA Sequence Binding Factor 1 (GRSF1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GRSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 30G49

Product Details anti-GRSF1 Antibody

Purpose: GRSF1 Antibody / G-rich RNA sequence-binding factor 1

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human GRSF1 was used as the immunogen for the GRSF1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GRSF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the GRSF1 antibody at -20oC.

Target Details for GRSF1

Target: GRSF1 (G-Rich RNA Sequence Binding Factor 1 (GRSF1))

Alternative Name: GRSF1

Background: GRSF1 antibody is designed to detect G-rich RNA sequence-binding factor 1, a multifunctional RNA-binding protein localized primarily to the mitochondria and nucleolus. GRSF1 plays a critical role in RNA metabolism, including RNA stabilization, processing, and translation. It is particularly important for mitochondrial RNA handling, where it binds G-rich elements and supports the maturation of mitochondrial transcripts required for oxidative phosphorylation. As a result, GRSF1 is an essential factor for maintaining mitochondrial protein synthesis and energy production.

GRSF1 antibody is widely employed in studies of RNA biology, mitochondrial function, and stress responses. Disruption of GRSF1 has been associated with impaired mitochondrial gene expression and respiratory chain defects, leading to compromised energy metabolism. Researchers use GRSF1 antibody to track changes in expression and localization under different cellular conditions, including oxidative stress and disease models that affect mitochondrial integrity.

Applications of GRSF1 antibody include western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, the antibody specifically recognizes GRSF1 protein bands, allowing for accurate comparisons of expression levels across experimental conditions. Immunohistochemistry provides tissue-level localization, while immunofluorescence highlights its mitochondrial distribution patterns in cultured cells. These techniques allow researchers to connect RNA-binding activity with mitochondrial homeostasis and gene expression.

GRSF1 also functions in the nucleolus, where it contributes to ribosomal RNA processing. This dual localization underscores its multifunctional role in RNA biology, linking mitochondrial gene expression with nuclear RNA processing pathways. By using GRSF1 antibody, researchers gain a powerful tool for exploring these interconnected processes.

In disease contexts, GRSF1 expression has been implicated in cancer and neurodegeneration. Altered RNA-binding activity may disrupt translation and contribute to disease pathology. Additionally, its role in mitochondrial maintenance makes it highly relevant for disorders involving energy metabolism and oxidative stress. Monitoring GRSF1 with specific antibodies allows for mechanistic studies that explore how RNA-binding proteins influence disease pathways.

GRSF1 antibody from NSJ Bioreagents offers reliable detection across multiple experimental methods, ensuring accurate analysis of this essential RNA-binding factor. Its proven specificity supports detailed investigations of RNA processing, mitochondrial function, and cellular stress adaptation.

UniProt: Q12849