Golgi Marker antibody (CF®488A)

Catalog No. ABIN7880922

The Mouse Monoclonal anti-Golgi Marker antibody is suitable to detect Golgi Marker in samples from Human. It has been validated for FACS and IF.

Quick Overview for Golgi Marker antibody (CF®488A) (ABIN7880922)

Target: Golgi Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: GLG1-970

Product Details

Purpose: Golgi Marker Antibody

Purification: Protein G affinity chromatography

Immunogen: The Golgi fraction from human liver cells was used as the immunogen for the Golgi Marker antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Golgi Marker antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 500 µL at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the Golgi Marker antibody at 2-8oC, protected from light.

Target Details

Target: Golgi Marker

Background: This MAb recognizes a protein of 134 kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. Highest levels are expressed in pancreas, skeletal muscle, placenta, heart, testis and ovary. It is also found in the kidney, liver, lung and brain. It is expressed in both adult and fetal tissues. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.

UniProt: Q92896