HCG beta antibody

Catalog No. ABIN7880961

The Mouse Monoclonal anti-HCG beta antibody is suitable to detect HCG beta in samples from Human. It has been validated for IHC (p).

Quick Overview for HCG beta antibody (ABIN7880961)

Target: HCG beta

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCG beta antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM529

Product Details anti-HCG beta Antibody

Purpose: HCG-beta Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant hCG beta protein was used as the immunogen for this hCG beta antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the HCG-beta antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the HCG-beta antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for HCG beta

Target: HCG beta

Alternative Name: HCG-beta

Background: HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. hCG mAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90 % and 60 % of cases respectively. 20 % of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.

UniProt: P01233