GRP94 antibody

Catalog No. ABIN7880987

This Rat Monoclonal antibody specifically detects GRP94 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for GRP94 antibody (ABIN7880987)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM249

Product Details anti-GRP94 Antibody

Purpose: gp96 Antibody / GRP94 / HSP90B1

Purification: Protein G affinity chromatography

Immunogen: Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the gp96 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the gp96 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the gp96 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: gp96

Background: Recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Gp96 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Gp96 has been briefly studied as a prognostic factor in breast cancer.

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades