HSPG2 antibody

Catalog No. ABIN7881008

This Rat Monoclonal antibody specifically detects HSPG2 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for HSPG2 antibody (ABIN7881008)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This HSPG2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM255

Product Details anti-HSPG2 Antibody

Purpose: Anti-Perlecan Antibody (HSPG)

Purification: Protein G affinity chromatography

Immunogen: A murine EHS laminin preparation was used as the immunogen for the anti-Perlecan antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the anti-Perlecan antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Perlecan antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Alternative Name: Perlecan

Background: This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism