Recombinant Histone H1 antibody

Catalog No. ABIN7881062

This Rabbit Monoclonal antibody specifically detects Histone H1 in WB, IF, FACS and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant Histone H1 antibody (ABIN7881062)

Target: Histone H1

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AE-4

Product Details anti-Histone H1 Antibody

Purpose: Recombinant Histone H1 Antibody

Purification: Protein A affinity chromatography

Immunogen: Nuclei of human leukemia biopsy cells were used as the immunogen for this recombinant Histone H1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Histone H1

Target: Histone H1

Background: Please note that this antibody is a recombinant rabbit version of original anti-histone H1 antibody (clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, this recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a pure preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.

UniProt: P07305