Recombinant MVP antibody

Catalog No. ABIN7881541

The Mouse Monoclonal anti-MVP antibody is suitable to detect MVP in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Recombinant MVP antibody (ABIN7881541)

Target: MVP (Major Vault Protein (MVP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MVP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: R1032

Product Details anti-MVP Antibody

Purpose: Recombinant MVP Antibody / Major Vault Protein

Purification: Protein A/G affinity

Immunogen: Proteins precipitated from human breast cancer MCF-7 cells were used as the immunogen for the recombinant MVP antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the recombinant MVP antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the recombinant MVP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for MVP

Target: MVP (Major Vault Protein (MVP))

Alternative Name: MVP

Background: Recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

UniProt: Q14764