MVP antibody

Catalog No. ABIN7881542

The Mouse Monoclonal anti-MVP antibody (Clone SPM280) (ABIN7881542) specifically detects MVP in IHC (p). The antibody is reactive with Human samples.

Quick Overview for MVP antibody (ABIN7881542)

Target: MVP (Major Vault Protein (MVP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MVP antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM280

Product Details anti-MVP Antibody

Purpose: MVP Antibody

Purification: Protein G affinity chromatography

Immunogen: Proteins precipitated from human breast cancer MCF-7 cells were used as the immunogen for the MVP antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the MVP antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the MVP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MVP

Target: MVP (Major Vault Protein (MVP))

Alternative Name: MVP

Background: Recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

UniProt: Q14764