Recombinant NMI antibody

Catalog No. ABIN7881750

This Rabbit Monoclonal antibody specifically detects NMI in WB, IHC, IF, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for Recombinant NMI antibody (ABIN7881750)

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NMI antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 30N95

Product Details anti-NMI Antibody

Purpose: NMI Antibody / N myc and STAT interactor

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human NMI was used as the immunogen for the NMI antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NMI antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the NMI antibody at -20oC.

Target Details for NMI

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Alternative Name: NMI

Background: NMI antibody detects N myc and STAT interactor, a coactivator protein encoded by the NMI gene. NMI interacts with transcription factors such as STATs and c-Myc, enhancing their transcriptional activity. It plays roles in cytokine signaling, oncogene regulation, and immune responses. By linking signal transduction to gene expression, NMI contributes to cellular responses to external cues.

NMI antibody is widely used in immunology, oncology, and signal transduction research. NMI expression is induced by interferons and other cytokines, where it modulates STAT mediated transcription. It also interacts with c-Myc and N-Myc, affecting oncogenic transcriptional programs. By detecting NMI, researchers can explore how transcriptional coactivators regulate immune signaling and cancer pathways.

In western blot assays, NMI antibody identifies protein bands of expected size in cytokine stimulated cells. Immunohistochemistry reveals expression in immune tissues, while immunofluorescence highlights nuclear and cytoplasmic distribution depending on activation state. These applications provide robust tools for analyzing NMI activity in signaling networks.

NMI has been implicated in tumorigenesis. Its expression is elevated in breast and cervical cancers, where it interacts with oncogenes and alters signaling outcomes. Conversely, NMI may also play tumor suppressive roles in certain contexts, reflecting its complex regulation. By applying NMI antibody, scientists can dissect the dual roles of this coactivator in cancer biology.

NMI also regulates antiviral responses and inflammation by modulating STAT signaling downstream of interferons. This positions NMI as a bridge between innate immunity and transcriptional responses. Its interactions with multiple transcription factors highlight its versatility in shaping gene expression outcomes.

NMI antibody from NSJ Bioreagents offers reliable specificity for investigating transcriptional regulation and immune signaling. Its performance across multiple assays supports accurate detection of NMI in basic and disease related research.

UniProt: Q13287