Recombinant NSDHL antibody

Catalog No. ABIN7881756

This Rabbit Monoclonal antibody specifically detects NSDHL in WB and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Recombinant NSDHL antibody (ABIN7881756)

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NSDHL antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 30N66

Product Details anti-NSDHL Antibody

Purpose: NSDHL Antibody / NADP-dependent steroid dehydrogenase-like protein

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human NSDHL was used as the immunogen for the NSDHL antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NSDHL antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the NSDHL antibody at -20oC.

Target Details for NSDHL

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Alternative Name: NSDHL

Background: NSDHL antibody is designed to detect NAD(P)-dependent steroid dehydrogenase-like protein, an essential enzyme of the cholesterol biosynthetic pathway. NSDHL is localized to the endoplasmic reticulum membrane and functions as a C-4 sterol dehydrogenase, catalyzing the removal of methyl groups from sterol intermediates. This enzymatic activity is necessary for producing cholesterol and other sterols that are critical for membrane structure, signaling, and developmental processes.

NSDHL antibody is widely used in studies of metabolism, developmental biology, and genetic disorders. Mutations in the NSDHL gene are associated with CHILD syndrome (congenital hemidysplasia with ichthyosiform erythroderma and limb defects), an X-linked dominant disorder characterized by skin abnormalities and limb malformations. Detecting NSDHL with specific antibodies enables researchers to evaluate expression and function in disease models and clinical studies.

Applications of NSDHL antibody include western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, NSDHL antibody detects the protein with high specificity, confirming its expression across cell and tissue samples. Immunohistochemistry reveals distribution patterns in tissues involved in sterol metabolism, while immunofluorescence highlights its endoplasmic reticulum localization in cultured cells. These experimental approaches provide important insights into cholesterol biosynthesis at the molecular level.

NSDHL plays a vital role in cholesterol metabolism, and dysregulation of its activity has broader implications for human health. Abnormal sterol accumulation contributes to metabolic disease, cardiovascular dysfunction, and neurodevelopmental abnormalities. Using NSDHL antibody, researchers can track enzyme activity and explore how disruptions in cholesterol biosynthesis impact cellular and systemic function.

Beyond inherited disorders, NSDHL has been implicated in cancer biology. Cholesterol metabolism supports rapid tumor growth, and enzymes in the biosynthetic pathway are increasingly recognized as potential therapeutic targets. Monitoring NSDHL expression with antibodies allows investigators to connect sterol metabolism with oncogenic processes, highlighting new avenues for intervention.

NSDHL antibody from NSJ Bioreagents is a reliable reagent for studying sterol metabolism, developmental genetics, and cholesterol-related disease. Its specificity ensures accurate detection in multiple research settings, supporting basic and translational studies alike.

UniProt: Q15738