Recombinant NDUFB4 antibody

Catalog No. ABIN7881759

This Rabbit Monoclonal antibody specifically detects NDUFB4 in WB, IF, IHC and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Recombinant NDUFB4 antibody (ABIN7881759)

Target: NDUFB4 (NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 4, 15kDa (NDUFB4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NDUFB4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 30N45

Product Details anti-NDUFB4 Antibody

Purpose: NDUFB4 Antibody / NADH dehydrogenase ubiquinone 1 beta subcomplex subunit 4

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human NDUFB4 was used as the immunogen for the NDUFB4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NDUFB4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the NDUFB4 antibody at -20oC.

Target Details for NDUFB4

Target: NDUFB4 (NADH Dehydrogenase (Ubiquinone) 1 beta Subcomplex, 4, 15kDa (NDUFB4))

Alternative Name: NDUFB4

Background: NDUFB4 antibody detects NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, encoded by the NDUFB4 gene. This protein is a non catalytic accessory subunit of mitochondrial Complex I, the first enzyme complex of the oxidative phosphorylation system. Complex I catalyzes the transfer of electrons from NADH to ubiquinone, coupled with proton translocation across the inner mitochondrial membrane. NDUFB4 antibody provides researchers with an essential reagent for studying mitochondrial respiratory function, bioenergetics, and disease mechanisms.

Although NDUFB4 does not directly participate in electron transfer, it contributes to the structural stability and assembly of Complex I. Research using NDUFB4 antibody has shown that accessory subunits help maintain the integrity of the complex, ensuring efficient electron flow and proton pumping. Loss or dysfunction of accessory proteins like NDUFB4 destabilizes Complex I, leading to reduced oxidative phosphorylation and ATP production. This highlights the importance of even non catalytic subunits in respiratory chain function.

Mutations and defects in Complex I subunits, including NDUFB4, are associated with mitochondrial diseases such as Leigh syndrome, encephalopathy, and mitochondrial myopathy. Research using NDUFB4 antibody has demonstrated that absence or reduction of NDUFB4 impairs assembly of Complex I, leading to decreased activity and energy deficiency. Such dysfunctions contribute to neuromuscular symptoms, developmental delays, and metabolic acidosis. Because mitochondrial disease mechanisms are complex, NDUFB4 antibody serves as an important tool for dissecting assembly defects and functional outcomes.

In cancer, altered expression of Complex I subunits including NDUFB4 has been observed. Tumor cells often exhibit metabolic reprogramming, favoring glycolysis over oxidative phosphorylation. However, subsets of tumors rely on intact mitochondrial respiration, making Complex I subunits potential biomarkers and therapeutic targets. Studies with NDUFB4 antibody have shown correlations between expression levels and tumor progression, underscoring its relevance in oncology. Beyond cancer, mitochondrial dysfunction involving NDUFB4 contributes to neurodegenerative diseases, metabolic disorders, and aging related decline.

NDUFB4 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein abundance in mitochondria rich tissues such as brain, heart, and muscle. Immunohistochemistry highlights tissue distribution, while immunofluorescence demonstrates mitochondrial localization using colocalization with markers such as COX IV. These approaches ensure that NDUFB4 antibody is useful in both clinical and basic mitochondrial research.

By supplying validated NDUFB4 antibody reagents, NSJ Bioreagents supports research into oxidative phosphorylation, mitochondrial disease, and cancer biology. Detection of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 enables researchers to explore how Complex I subunits maintain cellular energy production and contribute to disease when impaired.

UniProt: O95168