NEFH antibody

Catalog No. ABIN7881801

This Mouse Monoclonal antibody specifically detects NEFH in IHC (p). It exhibits reactivity toward Human.

Quick Overview for NEFH antibody (ABIN7881801)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM203

Product Details anti-NEFH Antibody

Purpose: Anti-NF-H Antibody

Purification: Protein G affinity chromatography

Immunogen: The Triton-X 100 insoluble protein fraction from rat brain was used as the immunogen for this anti-NF-H antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the anti-NF-H antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-NF-H antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: NF-H

Background: This mAb reacts with a 200 kDa protein, identified as heavy subunit of neurofilaments. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

UniProt: P12036