NFIA antibody

Catalog No. ABIN7881835

The Mouse Monoclonal anti-NFIA antibody is suitable to detect NFIA in samples from Human. It has been validated for WB, IF, FACS and IHC (p).

Quick Overview for NFIA antibody (ABIN7881835)

Target: NFIA (Nuclear Factor I/A (NFIA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NFIA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PCRP-NFIA-2C6

Product Details anti-NFIA Antibody

Purpose: NFIA Antibody / Nuclear Factor 1 A

Purification: Protein A/G affinity

Immunogen: A recombinant protein fragment was used as the immunogen for the NFIA antibody.

Isotype: IgG2c

Application Details

Application Notes: Optimal dilution of the NFIA antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the NFIA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for NFIA

Target: NFIA (Nuclear Factor I/A (NFIA))

Alternative Name: NFIA

Background: Nuclear factor (NF) proteins are a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, -globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.

UniProt: Q12857