Recombinant NOLC1 antibody

Catalog No. ABIN7881847

The Rabbit Monoclonal anti-NOLC1 antibody is suitable to detect NOLC1 in samples from Human. It has been validated for WB, IHC, IF and ICC.

Quick Overview for Recombinant NOLC1 antibody (ABIN7881847)

Target: NOLC1 (Nucleolar and Coiled-Body Phosphoprotein 1 (NOLC1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NOLC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 30N31

Product Details anti-NOLC1 Antibody

Purpose: NOLC1 Antibody / Nucleolar and coiled-body phosphoprotein 1 / NOPP140

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human NOLC1 was used as the immunogen for the NOLC1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NOLC1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the NOLC1 antibody at -20oC.

Target Details for NOLC1

Target: NOLC1 (Nucleolar and Coiled-Body Phosphoprotein 1 (NOLC1))

Alternative Name: NOLC1

Background: NOLC1 antibody detects nucleolar and coiled-body phosphoprotein 1, encoded by the NOLC1 gene. This protein is also called NOPP140, nuclear phosphoprotein 140, and hNOPP140. NOLC1 is a nucleolar protein that shuttles between the nucleolus and cytoplasm, functioning as a phosphoprotein involved in ribosome biogenesis, rRNA transcription, and nucleolar organization. Structurally, NOLC1 contains multiple serine-rich regions that undergo phosphorylation, regulating its localization and interactions with RNA polymerase I and ribosomal proteins.

NOLC1 antibody is widely applied in cell biology, cancer research, and nucleolar studies. By detecting NOLC1, researchers can investigate how the nucleolus coordinates ribosome production and stress responses. The protein is highly expressed in proliferating cells, linking it to growth and transformation. Dysregulated NOLC1 expression has been reported in cancers, where nucleolar function is often enhanced to meet elevated translational demands.

Applications of NOLC1 antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylated and unphosphorylated isoforms, immunohistochemistry maps expression in tumors, and immunofluorescence highlights nucleolar localization and dynamics. These approaches provide powerful tools for studying nucleolar biology in health and disease.

NOLC1 interacts with RNA polymerase I transcription machinery, fibrillarin, and nucleolin, coordinating rRNA transcription and processing. It also functions in nucleolar stress responses, where its relocalization signals cellular stress or DNA damage. By applying NOLC1 antibody, scientists can study how nucleolar function integrates with signaling pathways in cancer, aging, and neurodegeneration.

NOLC1 mutations and expression changes contribute to oncogenesis. Overexpression correlates with tumor growth, while knockdown reduces proliferation. Because nucleolar size and activity are biomarkers of cancer, NOLC1 detection provides diagnostic and prognostic potential. NSJ Bioreagents offers NOLC1 antibody with validated specificity, ensuring reliable performance in nucleolar and cancer research.

UniProt: Q14978

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling