Recombinant HRPT2 antibody

Catalog No. ABIN7881901

The Rabbit Monoclonal anti-HRPT2 antibody is suitable to detect HRPT2 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for Recombinant HRPT2 antibody (ABIN7881901)

Target: HRPT2 (CDC73) (Parafibromin (CDC73))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HRPT2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 31C54

Product Details anti-HRPT2 Antibody

Purpose: CDC73 Antibody / Cell division cycle protein 73

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human HRPT2 / CDC73 was used as the immunogen for the CDC73 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CDC73 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the CDC73 antibody at -20oC.

Target Details for HRPT2

Target: HRPT2 (CDC73) (Parafibromin (CDC73))

Alternative Name: CDC73

Background: CDC73 antibody detects cell division cycle protein 73, a tumor suppressor encoded by the CDC73 gene. CDC73 is also known as parafibromin and functions as a subunit of the PAF1 transcriptional regulatory complex. Through its association with RNA polymerase II, CDC73 regulates transcription elongation, histone modification, and RNA processing. Its tumor suppressor activity is critical for preventing uncontrolled cell growth and maintaining genomic integrity.

CDC73 antibody is widely applied in cancer research, molecular biology, and genetic studies. Mutations in CDC73 are associated with hyperparathyroidism-jaw tumor syndrome and parathyroid carcinoma. By detecting CDC73 expression, researchers can investigate its role in tumor suppression and transcriptional control. Loss of CDC73 expression serves as a diagnostic marker for parathyroid malignancy and supports studies of endocrine tumors.

Western blot assays reveal CDC73 protein in nuclear extracts, while immunohistochemistry highlights nuclear localization in normal tissues and its loss in tumor samples. Immunofluorescence provides high-resolution visualization of parafibromin within nuclear transcriptional complexes. These applications make CDC73 antibody valuable for both research and diagnostic contexts.

CDC73 also regulates histone methylation and chromatin remodeling through its interaction with the PAF1 complex. This regulation influences expression of genes involved in cell cycle, apoptosis, and development. By applying CDC73 antibody, scientists can study how transcriptional regulation by parafibromin contributes to normal growth and cancer prevention.

CDC73 antibody from NSJ Bioreagents provides strong specificity for analyzing tumor suppressor function and transcriptional regulation. Its performance across applications ensures accurate results for basic research, oncology, and clinical pathology.

UniProt: Q6P1J9

Pathways: Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance