Recombinant PMF1 antibody

Catalog No. ABIN7881994

The Rabbit Monoclonal anti-PMF1 antibody is suitable to detect PMF1 in samples from Human, Mouse and Rat. It has been validated for WB and IP.

Quick Overview for Recombinant PMF1 antibody (ABIN7881994)

Target: PMF1 (Polyamine Modulated Factor 1 (PMF1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PMF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 30P63

Product Details anti-PMF1 Antibody

Purpose: PMF1 Antibody / Polyamine modulated factor 1

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human PMF1 was used as the immunogen for the PMF1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PMF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the PMF1 antibody at -20oC.

Target Details for PMF1

Target: PMF1 (Polyamine Modulated Factor 1 (PMF1))

Alternative Name: PMF1

Background: PMF1 antibody detects Polyamine modulated factor 1, encoded by the PMF1 gene. Polyamine modulated factor 1 is a nuclear protein that functions as a transcriptional cofactor, particularly in regulating the activity of the transcription factor Nrf2 and the antioxidant response element pathway. PMF1 antibody provides researchers with a critical reagent to study oxidative stress, transcriptional regulation, and cancer biology.

Polyamine modulated factor 1 was originally identified for its ability to influence the binding of transcription factors to DNA in the presence of polyamines. Research using PMF1 antibody has demonstrated that it plays an essential role in regulating gene expression by modulating Nrf2 activity and controlling antioxidant response elements. This pathway is central to cellular defense against oxidative damage and environmental stressors, and PMF1 activity is critical in fine-tuning this response.

Beyond its role in transcription, PMF1 has been associated with chromatin organization. Studies with PMF1 antibody have shown that it interacts with histone modifying enzymes, suggesting a role in epigenetic regulation. By influencing both transcription factors and chromatin state, Polyamine modulated factor 1 integrates multiple layers of gene expression control. These findings expand its importance beyond a cofactor to a regulator of genome activity.

PMF1 has also been implicated in cancer. Research using PMF1 antibody has revealed altered expression in several tumor types, including lung, colon, and liver cancers. Elevated levels of PMF1 may enhance antioxidant defenses in tumor cells, promoting survival under stress conditions. Conversely, reduced expression may impair cellular responses to oxidative injury. These dual roles highlight its potential as a biomarker and therapeutic target.

PMF1 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects endogenous protein levels, immunohistochemistry highlights nuclear localization in tissue samples, and immunofluorescence demonstrates dynamic changes after oxidative stress. These applications make PMF1 antibody indispensable for transcription and stress research.

By supplying validated PMF1 antibody reagents, NSJ Bioreagents supports research into transcriptional regulation, oxidative stress, and cancer. Detection of Polyamine modulated factor 1 provides insight into how nuclear cofactors integrate environmental signals and gene expression.

UniProt: Q6P1K2