Recombinant PREB antibody

Catalog No. ABIN7882037

The Rabbit Monoclonal anti-PREB antibody (Clone 29P71) (ABIN7882037) specifically detects PREB in WB, IHC, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for Recombinant PREB antibody (ABIN7882037)

Target: PREB (Prolactin Regulatory Element Binding (PREB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PREB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 29P71

Product Details anti-PREB Antibody

Purpose: PREB Antibody / Prolactin regulatory element binding protein

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PREB was used as the immunogen for the PREB antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PREB antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the PREB antibody at -20oC.

Target Details for PREB

Target: PREB (Prolactin Regulatory Element Binding (PREB))

Alternative Name: PREB

Background: PREB antibody detects prolactin regulatory element binding protein, a transcription factor involved in gene regulation and vesicular trafficking. PREB contains WD-repeat domains that facilitate protein-protein interactions, allowing it to function as a transcriptional regulator and vesicle-associated factor. It is expressed in a variety of tissues, with high levels in brain, pituitary, and pancreas, underscoring its multifunctional roles in development and physiology.

Research using PREB antibody has revealed two primary functions of the protein. First, PREB binds to promoter elements of the prolactin gene, regulating hormone expression in the anterior pituitary. By influencing prolactin secretion, PREB impacts reproductive biology, lactation, and endocrine homeostasis. Second, PREB is associated with vesicular transport machinery, where it facilitates protein trafficking between the endoplasmic reticulum and Golgi apparatus. This dual role positions PREB as a regulator of both transcription and intracellular trafficking.

In developmental biology, PREB has been shown to be essential for embryogenesis and organogenesis. Knockout models reveal that PREB deficiency leads to embryonic lethality, indicating its necessity for proper gene regulation and cellular organization. Heterozygous models display neurological and metabolic abnormalities, linking PREB to central nervous system development and energy balance.

PREB has also been studied in the context of neurological and metabolic disorders. Altered PREB expression affects neuroendocrine regulation and insulin secretion. Dysregulation of vesicular trafficking involving PREB has implications for protein misfolding diseases and diabetes. In cancer research, PREB expression changes have been linked to tumor progression, although its exact role remains under investigation.

Antibodies against PREB are validated for western blot, immunohistochemistry, and immunofluorescence. These reagents allow researchers to study nuclear localization, promoter occupancy, and trafficking-related functions. Clone-based antibodies ensure specificity and reproducibility across experimental systems.

NSJ Bioreagents supplies this PREB antibody for research into transcriptional regulation, neuroendocrine biology, and vesicular trafficking.

UniProt: Q9HCU5

Pathways: ER-Nucleus Signaling