Recombinant PREPL antibody

Catalog No. ABIN7882048

The Rabbit Monoclonal anti-PREPL antibody is suitable to detect PREPL in samples from Human, Mouse and Rat. It has been validated for WB, IF, IHC, ICC and IP.

Quick Overview for Recombinant PREPL antibody (ABIN7882048)

Target: PREPL (Prolyl Endopeptidase Like Protein (PREPL))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PREPL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 30P79

Product Details anti-PREPL Antibody

Purpose: PREPL Antibody / Prolyl endopeptidase-like protein

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human PREPL was used as the immunogen for the PREPL antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PREPL antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the PREPL antibody at -20oC.

Target Details for PREPL

Target: PREPL (Prolyl Endopeptidase Like Protein (PREPL))

Alternative Name: PREPL

Background: PREPL antibody detects Prolyl endopeptidase-like protein, encoded by the PREPL gene. This protein belongs to the prolyl oligopeptidase family of serine peptidases, but unlike many of its relatives, PREPL is thought to have limited or absent catalytic activity. Instead, it is believed to function as a scaffolding or regulatory protein influencing peptide processing, vesicle trafficking, and synaptic transmission. PREPL antibody provides an important reagent for studying rare genetic disease, peptide metabolism, and neurological function.

Mutations in PREPL cause a rare genetic disorder known as hypotonia-cystinuria syndrome, characterized by neonatal hypotonia, muscle weakness, and cystinuria due to loss of function in both PREPL and SLC3A1. Research using PREPL antibody has revealed that absence of Prolyl endopeptidase-like protein disrupts normal vesicle trafficking and neurotransmitter release, contributing to hypotonia and neurological deficits. Although the precise enzymatic role of PREPL remains unclear, its presence in synaptic vesicles suggests it contributes to regulated peptide handling and signaling in the nervous system.

Beyond hypotonia-cystinuria syndrome, alterations in PREPL expression have been studied in broader contexts such as growth, appetite regulation, and endocrine function. Research with PREPL antibody has demonstrated its expression in brain and endocrine tissues, where it may modulate hormone release and metabolic signaling. Some studies suggest that PREPL deficiency influences growth hormone secretion, highlighting its potential role in development. These findings make PREPL an interesting target in both rare disease and general physiology.

PREPL antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting demonstrates tissue specific expression, especially in brain and muscle. Immunohistochemistry highlights its distribution in endocrine and neuronal tissues, while immunofluorescence confirms subcellular localization to vesicular structures. Together, these methods provide insights into PREPL's role in vesicle biology and signaling. Functional studies supported by PREPL antibody include knockout models where absence of PREPL reveals physiological consequences at cellular and organismal levels.

By supplying validated PREPL antibody reagents, NSJ Bioreagents supports research into neurobiology, peptide regulation, and rare disease. Detection of Prolyl endopeptidase-like protein provides an essential tool for understanding how peptide handling and vesicle trafficking affect health and disease.

UniProt: Q4J6C6