Recombinant PKC antibody

Catalog No. ABIN7882075

The Rabbit Monoclonal anti-PKC antibody is suitable to detect PKC in samples from Human, Rat and Mouse. It has been validated for WB, IF, ICC, FACS and IP.

Quick Overview for Recombinant PKC antibody (ABIN7882075)

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PKC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 31P91

Product Details anti-PKC Antibody

Purpose: PKC Antibody / Protein kinase C

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PKC was used as the immunogen for the PKC antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PKC antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the PKC antibody at -20oC.

Target Details for PKC

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Alternative Name: PKC

Background: PKC antibody detects Protein kinase C (PKC), a family of serine/threonine kinases that play critical roles in cellular signal transduction, gene expression, and membrane receptor regulation. The UniProt recommended name is Protein kinase C. This enzyme family mediates diverse biological responses by phosphorylating target proteins involved in proliferation, apoptosis, differentiation, and cytoskeletal organization. PKC enzymes are key downstream effectors of diacylglycerol (DAG) and calcium signaling pathways, serving as central hubs that integrate extracellular signals into intracellular responses.

Protein kinase C includes multiple isoforms that can be broadly divided into three classes: conventional (alpha, beta, gamma), novel (delta, epsilon, eta, theta), and atypical (zeta, iota/lambda). Each isoform differs in its activation requirements and tissue distribution. Conventional isoforms require both calcium and DAG for activation, whereas novel isoforms are calcium-independent but DAG-dependent. Atypical isoforms do not require either calcium or DAG, responding instead to other lipid mediators. These differences allow PKC family members to finely tune signaling cascades in a context-dependent manner.

Functionally, PKC regulates numerous processes such as receptor desensitization, ion channel modulation, transcription factor activation, and cytoskeletal remodeling. It is activated by various stimuli including growth factors, hormones, and neurotransmitters through phospholipase C-mediated generation of DAG. Once activated, PKC translocates to cellular membranes where it phosphorylates specific substrates, leading to altered protein activity and gene expression. In neurons, PKC modulates synaptic plasticity, while in cardiac and smooth muscle it influences contraction and metabolism.

The PKC gene family is expressed across nearly all tissues, with particular enrichment in brain, heart, and skeletal muscle. Dysregulation of PKC activity has been linked to pathological conditions such as cancer, diabetes, neurodegeneration, and cardiovascular disease. In oncology, PKC can act as either a tumor promoter or suppressor depending on the isoform and cellular context. Therefore, antibodies targeting PKC are valuable tools for investigating its expression patterns, activation states, and downstream signaling pathways.

PKC antibody is useful in immunohistochemistry, immunofluorescence, and related assays to assess protein localization, phosphorylation-dependent signaling, and isoform-specific expression. By detecting total PKC or specific subtypes, researchers can study its role in signal transduction and disease progression. NSJ Bioreagents provides PKC antibody reagents optimized for research in kinase signaling, cellular communication, and phosphoregulation studies.

UniProt: P05129

Pathways: Positive Regulation of Response to DNA Damage Stimulus