Recombinant PRPF4 antibody

Catalog No. ABIN7882101

This Rabbit Monoclonal antibody specifically detects PRPF4 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant PRPF4 antibody (ABIN7882101)

Target: PRPF4 (PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PRPF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 30P60

Product Details anti-PRPF4 Antibody

Purpose: PRPF4 Antibody / Pre mRNA processing factor 4

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PRPF4 was used as the immunogen for the PRPF4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PRPF4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the PRPF4 antibody at -20oC.

Target Details for PRPF4

Target: PRPF4 (PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4))

Alternative Name: PRPF4

Background: PRPF4 antibody detects pre mRNA processing factor 4, a spliceosome associated protein encoded by the PRPF4 gene. PRPF4 is a component of the U4/U6.U5 tri snRNP complex, which is required for pre mRNA splicing. By stabilizing interactions between snRNPs, PRPF4 contributes to spliceosome assembly and catalytic activation. This function makes PRPF4 essential for gene expression and RNA processing in all eukaryotic cells.

PRPF4 antibody is widely used in molecular biology and genetic research. Mutations in PRPF4 are linked to retinitis pigmentosa, a degenerative eye disease characterized by progressive photoreceptor loss. By detecting PRPF4, researchers can study the molecular basis of splicing related diseases and investigate how splicing defects cause tissue specific pathology despite the ubiquitous nature of the spliceosome.

In western blot assays, a PRPF4 antibody detects protein bands of the expected size in nuclear extracts. Immunohistochemistry reveals PRPF4 expression in retina and other tissues, while immunofluorescence highlights nuclear localization in splicing speckles. These applications support detailed study of spliceosomal proteins in cells and tissues.

PRPF4 is critical for RNA splicing fidelity and gene expression regulation. Disruption of PRPF4 function alters transcriptome diversity and contributes to developmental abnormalities. Its role in retinitis pigmentosa underscores the importance of splicing factors in specialized tissues such as retina. By applying PRPF4 antibody, scientists can explore how mutations affect splicing and develop potential therapeutic strategies to restore RNA processing.

PRPF4 also interacts with kinases and other splicing regulators, linking it to signaling pathways that modulate spliceosome dynamics. These interactions suggest broader roles for PRPF4 in cell cycle control and stress responses. As a result, PRPF4 antibody is valuable for investigating how splicing integrates with cellular physiology.

PRPF4 antibody from NSJ Bioreagents provides strong specificity for studying spliceosomal function and RNA processing. Its proven reliability across applications ensures accurate detection of this essential splicing factor in basic and disease related research.

UniProt: O43172