Recombinant PRPF4 antibody

Catalog No. ABIN7882102

The Rabbit Monoclonal anti-PRPF4 antibody is suitable to detect PRPF4 in samples from Human. It has been validated for WB, IHC, IF, ICC, FACS and IP.

Quick Overview for Recombinant PRPF4 antibody (ABIN7882102)

Target: PRPF4 (PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PRPF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 30P52

Product Details anti-PRPF4 Antibody

Purpose: PRPF4 Antibody / Pre mRNA processing factor 4

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PRPF4 was used as the immunogen for the PRPF4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PRPF4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the PRPF4 antibody at -20oC.

Target Details for PRPF4

Target: PRPF4 (PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4))

Alternative Name: PRPF4

Background: PRPF4 antibody recognizes pre mRNA processing factor 4, a nuclear protein essential for spliceosome assembly and pre mRNA splicing. PRPF4 is a component of the U4 and U6 small nuclear ribonucleoproteins and is required for the formation of the U4 or U6 U5 tri small nuclear ribonucleoprotein complex. This complex is indispensable for pre mRNA splicing, a fundamental process that ensures accurate removal of introns and joining of exons to produce mature messenger RNA.

PRPF4 antibody is highly relevant for studies of gene expression regulation and RNA biology. By detecting PRPF4, researchers can investigate how spliceosome components assemble and how splicing is regulated under different physiological conditions. Disruption of PRPF4 function impairs RNA processing and leads to widespread changes in gene expression, highlighting its importance in cell biology.

The antibody is suitable for western blotting, immunohistochemistry, immunofluorescence, and other molecular biology applications. In western blot assays, PRPF4 antibody recognizes nuclear protein bands, confirming expression across tissue and cell samples. Immunohistochemistry provides tissue localization, often highlighting nuclear distribution in actively transcribing cells. Immunofluorescence further resolves subnuclear localization and colocalization with other spliceosomal proteins.

Mutations in PRPF4 are linked to retinitis pigmentosa, a degenerative retinal disorder characterized by progressive vision loss. This connection underscores the physiological importance of splicing factors beyond general RNA metabolism. PRPF4 antibody therefore provides a valuable reagent for studies of inherited retinal disease as well as broader investigations of splicing related disorders.

Splicing dysregulation is also increasingly recognized in cancer and neurodegeneration. Aberrant splicing contributes to oncogenic transformation, resistance to therapy, and progression of neurodegenerative diseases. Detecting PRPF4 expression with specific antibodies enables researchers to connect splicing factor function with disease mechanisms and to evaluate therapeutic interventions targeting the spliceosome.

PRPF4 antibody from NSJ Bioreagents offers strong performance across assay types, ensuring accurate detection of this critical spliceosomal protein. It is a powerful tool for molecular biology, disease research, and therapeutic development focused on RNA regulation.

UniProt: O43172