Recombinant RASEF antibody

Catalog No. ABIN7882137

This Rabbit Monoclonal antibody specifically detects RASEF in WB, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant RASEF antibody (ABIN7882137)

Target: RASEF (RAS and EF Hand Domain Containing (RASEF))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RASEF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 30R48

Product Details anti-RASEF Antibody

Purpose: RASEF Antibody / RAB45 / Ras and EF-hand domain-containing protein

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human RAB45 was used as the immunogen for the RASEF antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RASEF antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the RASEF antibody at -20oC.

Target Details for RASEF

Target: RASEF (RAS and EF Hand Domain Containing (RASEF))

Alternative Name: RASEF

Background: RASEF antibody detects Ras and EF hand domain containing protein, also known as RAB45, encoded by the RASEF gene. RASEF is a member of the Rab family of small GTP binding proteins with unique EF hand calcium binding motifs. This combination of domains links small GTPase activity with calcium regulated signaling, positioning RASEF as a multifunctional regulator of vesicular trafficking, cytoskeletal dynamics, and cell signaling. RASEF localizes to endosomal and cytoplasmic membranes, where it contributes to transport and signaling events.

RASEF antibody is widely applied in cancer research, cell biology, and immunology. RASEF has been identified as a tumor suppressor gene in uveal melanoma, where mutations or promoter hypermethylation reduce its expression. Conversely, altered RASEF activity has been observed in lung and breast cancer, suggesting context dependent functions. By detecting RASEF, researchers can investigate its dual roles in tumor biology and cell signaling.

Western blot assays detect RASEF protein in diverse tissue lysates. Immunohistochemistry maps expression in eye, lung, and immune tissues, while immunofluorescence highlights subcellular localization to endosomes and membranes. These methods provide robust tools for analyzing RASEF biology.

RASEF contributes to immune signaling through regulation of endosomal trafficking and receptor recycling. It may influence T cell activation, antigen presentation, and cytokine signaling by modulating endosomal compartments. Its calcium binding EF hand motifs suggest integration of calcium signaling with vesicle dynamics. By applying RASEF antibody, scientists can examine how small GTPases coordinate trafficking and signaling across biological systems.

Beyond immunity and cancer, RASEF has roles in ocular biology, where it is expressed in retinal tissues and linked to eye disease. It also contributes to cellular homeostasis through regulation of vesicle fusion and cytoskeletal remodeling. These diverse functions make RASEF antibody valuable for both basic science and translational research.

RASEF antibody from NSJ Bioreagents provides dependable specificity for detecting Ras and EF hand domain containing protein. Its performance across applications ensures accurate results in cancer biology, cell signaling, and immune regulation studies.

UniProt: Q8IZ41