RUVBL1 antibody

Catalog No. ABIN7882186

The Rabbit Polyclonal anti-RUVBL1 antibody is suitable to detect RUVBL1 in samples from Human. It has been validated for WB, FACS and IHC (p).

Quick Overview for RUVBL1 antibody (ABIN7882186)

Target: RUVBL1 (RuvB-Like 1 (E. Coli) (RUVBL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUVBL1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RUVBL1 Antibody

Purpose: RuvB-like 1 Antibody / RUVBL1 / TIP49A

Purification: Antigen affinity purified

Immunogen: Recombinant human protein was used as the immunogen for the RuvB-like 1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the RuvB-like 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the RuvB-like 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for RUVBL1

Target: RUVBL1 (RuvB-Like 1 (E. Coli) (RUVBL1))

Alternative Name: RuvB-like 1

Background: RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1 -CTNNB1 complex. High levels of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases such as polymyositis/dermatomyosistis and autoimmune hepatitis.

UniProt: Q9Y265

Pathways: Telomere Maintenance