Recombinant SAFB antibody

Catalog No. ABIN7882213

The Rabbit Monoclonal anti-SAFB antibody is suitable to detect SAFB in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ICC.

Quick Overview for Recombinant SAFB antibody (ABIN7882213)

Target: SAFB (Scaffold Attachment Factor B (SAFB))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SAFB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 30S09

Product Details anti-SAFB Antibody

Purpose: SAFB Antibody / Scaffold attachment factor B

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human SAFB was used as the immunogen for the SAFB antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SAFB antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the SAFB antibody at -20oC.

Target Details for SAFB

Target: SAFB (Scaffold Attachment Factor B (SAFB))

Alternative Name: SAFB

Background: SAFB antibody detects scaffold attachment factor B, a multifunctional nuclear protein encoded by the SAFB gene. SAFB belongs to a family of proteins that bind scaffold/matrix attachment regions of DNA, thereby influencing chromatin structure and transcriptional regulation. It contains RNA recognition motifs and domains for protein-protein interactions, linking it to RNA processing, transcriptional repression, and nuclear architecture.

SAFB antibody is widely applied in molecular biology, transcriptional regulation, and cancer research. SAFB associates with nuclear matrix elements and modulates expression of hormone responsive genes. It also functions as a corepressor of estrogen receptor alpha, impacting signaling pathways critical in breast cancer biology. By detecting SAFB, researchers can explore mechanisms of gene regulation at the interface of DNA, RNA, and protein interactions.

Western blot assays detect SAFB protein in nuclear extracts, while immunohistochemistry maps expression in hormone responsive tissues and tumors. Immunofluorescence highlights nuclear speckle localization, consistent with its role in transcriptional complexes. These applications enable detailed characterization of SAFB across tissues and conditions.

SAFB also contributes to stress response pathways. It regulates heat shock protein gene expression and participates in apoptosis and cell cycle control. Mutations or altered expression of SAFB have been linked to breast cancer, prostate cancer, and neurological disorders. By applying SAFB antibody, scientists can evaluate its diverse functions in gene regulation, hormone signaling, and disease pathogenesis.

SAFB antibody from NSJ Bioreagents provides reliable specificity for analyzing nuclear scaffold interactions and transcriptional repression. Its performance across multiple assays ensures accurate results in both research and diagnostic contexts.

UniProt: Q15424

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway