STUB1 antibody

Catalog No. ABIN7882378

The Mouse Monoclonal anti-STUB1 antibody is suitable to detect STUB1 in samples from Human and Mouse. It has been validated for WB.

Quick Overview for STUB1 antibody (ABIN7882378)

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This STUB1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1008CT12-4-1

Product Details anti-STUB1 Antibody

Purpose: STUB1 Antibody (C-Terminal Region)

Purification: Protein G purified

Immunogen: Recombinant human STUB1 was used as the immunogen for the STUB1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the STUB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the STUB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for STUB1

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Alternative Name: STUB1

Background: E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF- BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. [UniProt]

UniProt: Q9UNE7

Pathways: Regulation of Hormone Metabolic Process, Response to Water Deprivation